Pessa M E, Sitren H S, Copeland E M, Talbert J L, Schmidt G, Baumgartner T G
Department of Food Science, University of Florida, Gainesville 32611.
JPEN J Parenter Enteral Nutr. 1988 Jan-Feb;12(1):63-7. doi: 10.1177/014860718801200163.
A rat model was developed to study intraperitoneal (ip) dialysis as a means of total nutritional support. Rats (200 g) were implanted ip with a catheter device and connected to a rodent infusion assembly. An automated system exchanged 10-ml volumes of a 37 degrees C solution containing 10% dextrose, 2% amino acid solution plus micronutrients. Rats were adapted over 3 days to a schedule of 16 1-hr cycles/day, and continued on this regimen for another 4 days. Rats subjected to this program maintained similar body weight, nitrogen balance, plasma chemistries, and liver tests in comparison to control animals fed per os in isocaloric and isonitrogenous amounts. Efficiency of peritoneal absorption for both glucose and amino acid was 95%. Histological examination of intraabdominal organs revealed only mild inflammation. This model is applicable to studies involving nutritional support via the peritoneal cavity, a technique which may be of value in patients with sensitive fluid balances (cardiac, renal, or pulmonary failure).
为研究腹腔内（ip）透析作为全营养支持手段，建立了大鼠模型。将大鼠（200 g）腹腔内植入导管装置，并连接到啮齿动物输注组件。一个自动化系统以1小时为周期，每天交换16次10毫升含10%葡萄糖、2%氨基酸溶液及微量营养素的37℃溶液。大鼠在3天内适应每天16个1小时周期的安排，并在此方案下再持续4天。与经口给予等热量和等氮量食物的对照动物相比，接受该方案的大鼠体重、氮平衡、血浆化学指标和肝脏检测结果相似。葡萄糖和氨基酸的腹膜吸收效率均为95%。腹腔内器官的组织学检查仅显示轻度炎症。该模型适用于涉及通过腹腔进行营养支持的研究，这一技术可能对液体平衡敏感的患者（心力衰竭、肾衰竭或肺衰竭）有价值。
