Pogorelova T N, Krukier I I, Gunko V O, Nikashina A A, Alliluev I A, Larichkin A V
Scientific-Research Institute of Obstetrics and Pediatrics of Rostov State Medical University, Rostov-on-Don, Russia.
Scientific-Research Institute of Obstetrics and Pediatrics of Rostov State Medical University, Rostov-on-Don, Russia; Academy of Biology and Biotechnology of the South Federal University, Rostov-on-Don, Russia.
Biomed Khim. 2019 Apr;65(3):245-250. doi: 10.18097/PBMC20196503245.
The content of vasoactive compounds and arachidonic acid in the placenta and amniotic fluid was studied in full-term (39-40 weeks) physiological pregnancy and preeclampsia (PE). The content of metabolites of nitric oxide (NOx), endothelin-1, thromboxane B2 (TxB2), prostacycline (PGI2) and arachidonic acid was estimated using spectrophotometric, immunoenzyme methods and gas-liquid chromatography. It was found that in PE the content of vasoconstrictors, of endothelin and TxB2, increased in the placenta and amniotic fluid, while the content of vasodilators, PGI2 and NOx decreased. Despite the same directionality of changes in both studied objects, the degree of changes differed and was more pronounced in the placenta. A direct or inverse correlative relationship was found between various vasoactive components (depending on their effect on vascular tone). In the case of arachidonic acid changes in its content in PE correlated with the level of vasoactive compounds, the source of which it is. The revealed differences in the ratio of vasoactive components obviously play a pathogenetic role in the development of PE and its subsequent complications.
研究了足月（39 - 40周）生理妊娠和子痫前期（PE）时胎盘和羊水中血管活性化合物及花生四烯酸的含量。使用分光光度法、免疫酶法和气液色谱法估算一氧化氮代谢物（NOx）、内皮素 - 1、血栓素B2（TxB2）、前列环素（PGI2）和花生四烯酸的含量。结果发现，在子痫前期，胎盘和羊水中血管收缩剂（内皮素和TxB2）的含量增加，而血管舒张剂（PGI2和NOx）的含量降低。尽管两个研究对象的变化方向相同，但变化程度不同，且在胎盘中更为明显。发现各种血管活性成分之间存在直接或反向的相关关系（取决于它们对血管张力的影响）。在花生四烯酸方面，子痫前期其含量的变化与血管活性化合物的水平相关，而花生四烯酸是这些血管活性化合物的来源。所揭示的血管活性成分比例差异显然在子痫前期及其后续并发症的发生发展中起致病作用。
