Hirata Bruna K S, Pedroso Amanda P, Machado Meira M F, Neto Nelson I P, Perestrelo Bruna O, de Sá Roberta D C C, Alonso-Vale Maria Isabel C, Nogueira Fernando N, Oyama Lila M, Ribeiro Eliane B, Tashima Alexandre K, Telles Monica M
Department of Biological Sciences, Universidade Federal de São Paulo-UNIFESP, Diadema, Brazil.
Department of Physiology, Universidade Federal de São Paulo-UNIFESP, São Paulo, Brazil.
Front Pharmacol. 2019 Jun 14;10:686. doi: 10.3389/fphar.2019.00686. eCollection 2019.
The rapid increase in the number of individuals with obesity, over the past four decades, is triggered by a number of complex interactions among factors. Despite the plethora of treatments available, side effects are commonly observed and, in this context, herbal medicines have been employed as an alternative form of therapy. extract (GbE) has been described as a promising new pharmacological approach to treat obesity. In order to better comprehend the mechanisms involved with this potential effect, the present study evaluated the effects of GbE treatment on diet-induced obese rats, focusing on the proteome and the oxidative stress defense system of visceral adipose tissue. After 14 days treatment, GbE significantly modulated 25 proteins. Retroperitoneal adipose tissue of treated animals exhibited higher amounts of proteins associated with adipogenesis (decorin), carbon metabolism and mitochondrial function (citrate synthase), and a concomitant reduction in adipocyte hypertrophy. In parallel, GbE down-regulated proteins involved in oxidative stress (peroxiredoxin) and the inflammatory response (complement C3, mast cell protease 1, and Ig gamma-2B chain C region). Moreover, also related to oxidative stress defense, GbE stimulated catalase activity, reduced malondialdehyde levels (lipid peroxidation indicator), and increased lactoylglutathione lyase levels. It was concluded that GbE acts as an antioxidant agent, and improved the proteome profile and oxidative stress response in the adipose tissue of diet-induced obese rats.
在过去的四十年里，肥胖个体数量的迅速增加是由多种因素之间的复杂相互作用引发的。尽管有大量可用的治疗方法，但副作用却很常见，在这种情况下，草药已被用作一种替代治疗形式。银杏叶提取物（GbE）已被描述为一种有前景的治疗肥胖的新药理学方法。为了更好地理解这种潜在作用所涉及的机制，本研究评估了GbE治疗对饮食诱导肥胖大鼠的影响，重点关注内脏脂肪组织的蛋白质组和氧化应激防御系统。经过14天的治疗，GbE显著调节了25种蛋白质。治疗动物的腹膜后脂肪组织中与脂肪生成相关的蛋白质（核心蛋白聚糖）、碳代谢和线粒体功能（柠檬酸合酶）含量更高，同时脂肪细胞肥大减少。与此同时，GbE下调了参与氧化应激的蛋白质（过氧化物酶）和炎症反应的蛋白质（补体C3、肥大细胞蛋白酶1和Igγ-2B链C区）。此外，同样与氧化应激防御相关，GbE刺激了过氧化氢酶活性，降低了丙二醛水平（脂质过氧化指标），并提高了乳酰谷胱甘肽裂解酶水平。得出的结论是，GbE作为一种抗氧化剂，改善了饮食诱导肥胖大鼠脂肪组织中的蛋白质组谱和氧化应激反应。
