Fournier J M, Hannon K, Moreau M, Karakawa W W, Vann W F
Unité du Choléra et des Vibrions, Institut Pasteur, Paris.
Ann Inst Pasteur Microbiol. 1987 Sep-Oct;138(5):561-7. doi: 10.1016/0769-2609(87)90041-x.
Clinical surveys have indicated the predominance of type 5 and type 8 capsular polysaccharides among clinical isolates of Staphylococcus aureus. The type 5 capsular polysaccharide was extracted from three clinical isolates of S. aureus grown on solid media, and purified by ion exchange chromatography and gel filtration. Chemical analysis showed that the type 5 capsular polysaccharide is composed of N-acetylfucosamine and N-acetylhexosaminuronic acid. Immunodiffusion and 13C nuclear magnetic resonance experiments showed that the type 5 capsular polysaccharide is both immunologically and chemically distinct from the type 8 capsular polysaccharide and the teichoic acid of S. aureus.
临床调查表明，在金黄色葡萄球菌的临床分离株中，5型和8型荚膜多糖占主导地位。从在固体培养基上生长的三株金黄色葡萄球菌临床分离株中提取5型荚膜多糖，并通过离子交换色谱法和凝胶过滤法进行纯化。化学分析表明，5型荚膜多糖由N-乙酰岩藻糖胺和N-乙酰己糖胺糖醛酸组成。免疫扩散和13C核磁共振实验表明，5型荚膜多糖在免疫学和化学上均与8型荚膜多糖及金黄色葡萄球菌的磷壁酸不同。
