Rocha Marcele Neves, Duarte Myrian Morato, Mansur Simone Brutman, Silva Bianca Daoud Mafra E, Pereira Thiago Nunes, Adelino Talita Émile Ribeiro, Giovanetti Marta, Alcantara Luis Carlos Junior, Santos Franciele Martins, Costa Victor Rodrigues de Melo, Teixeira Mauro Martins, Iani Felipe Campos de Melo, Costa Vivian Vasconcelos, Moreira Luciano Andrade
Mosquitos Vetores, IRR, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brazil.
Serviço de Virologia e Riquetsioses, Fundação Ezequiel Dias-LACEN, Belo Horizonte, MG, Brazil.
Gates Open Res. 2019 Apr 16;3:161. doi: 10.12688/gatesopenres.12903.2. eCollection 2019.
: Yellow fever outbreaks have re-emerged in Brazil during 2016-18, with mortality rates up to 30%. Although urban transmission has not been reported since 1942, the risk of re-urbanization of yellow fever is significant, as is present in most tropical and sub-tropical cities in the World and still remains the main vector of urban YFV. Although the YFV vaccine is safe and effective, it does not always reach populations at greatest risk of infection and there is an acknowledged global shortage of vaccine supply. The introgression of bacteria into mosquito populations is being trialed in several countries ( www.worldmosquito.org) as a biocontrol method against dengue, Zika and chikungunya. Here, we studied the ability of to reduce the transmission potential of mosquitoes for (YFV). Two recently isolated YFV (primate and human) were used to challenge field-derived wild-type and -infected ( Mel +) mosquitoes. The YFV infection status was followed for 7, 14 and 21 days post-oral feeding (dpf). The YFV transmission potential of mosquitoes was evaluated via nano-injection of saliva into uninfected mosquitoes or by inoculation in mice. We found that was able to significantly reduce the prevalence of mosquitoes with YFV infected heads and thoraces for both viral isolates. Furthermore, analyses of mosquito saliva, through indirect injection into naïve mosquitoes or via interferon-deficient mouse model, indicated was associated with profound reduction in the YFV transmission potential of mosquitoes (14dpf). Our results suggest that introgression could be used as a complementary strategy for prevention of urban yellow fever transmission, along with the human vaccination program.
2016 - 2018年期间，巴西再次出现黄热病疫情，死亡率高达30%。尽管自1942年以来尚未报告过城市传播情况，但黄热病重新在城市传播的风险很大，因为埃及伊蚊存在于世界上大多数热带和亚热带城市，并且仍然是城市黄热病毒（YFV）的主要传播媒介。尽管YFV疫苗安全有效，但它并不总能覆盖到感染风险最高的人群，而且全球公认存在疫苗供应短缺的问题。目前，几个国家正在试验将细菌导入蚊子种群（www.worldmosquito.org），作为一种针对登革热、寨卡病毒和基孔肯雅热的生物防治方法。在此，我们研究了埃及伊蚊携带沃尔巴克氏体对黄热病毒（YFV）传播潜力的降低能力。使用两种最近分离的YFV（灵长类和人类）毒株来感染从野外采集的野生型和携带沃尔巴克氏体（Mel +）的埃及伊蚊。在口服感染后7天、14天和21天跟踪YFV感染状况。通过将唾液纳米注射到未感染的蚊子体内或接种到小鼠体内来评估蚊子传播YFV的潜力。我们发现，对于两种病毒分离株，埃及伊蚊携带沃尔巴克氏体均能显著降低感染YFV的头部和胸部蚊子的患病率。此外，通过间接注射到未感染的蚊子体内或通过干扰素缺陷小鼠模型对蚊子唾液进行分析表明，埃及伊蚊携带沃尔巴克氏体与蚊子传播YFV的潜力显著降低有关（口服感染后14天）。我们的研究结果表明，导入沃尔巴克氏体可作为预防城市黄热病传播的补充策略，与人类疫苗接种计划一起使用。
