Yang J T, Saxton W M, Goldstein L S
Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138.
Proc Natl Acad Sci U S A. 1988 Mar;85(6):1864-8. doi: 10.1073/pnas.85.6.1864.
An antiserum that recognizes the heavy chain of Drosophila kinesin was used to isolate Drosophila cDNA clones. Immunoblot analysis of the proteolytic fragments of the protein produced by one of the cDNA clones has demonstrated that the cDNA clones encode the heavy chain of Drosophila kinesin. The in vitro-synthesized product of the largest cDNA comigrates with Drosophila kinesin heavy chain on NaDodSO4/polyacrylamide gels and binds to taxol-stabilized microtubules in the presence of the nonhydrolyzable analogue of ATP, 5'-adenylyl imidodiphosphate, but not in the presence of ATP or 0.1 M KCl. Analysis of the cDNA clones suggests that there is a single gene encoding kinesin heavy chain in Drosophila located at polytene chromosome position 53A. However, Southern hybridization analyses suggest the presence of related sequences in the Drosophila genome.
一种能识别果蝇驱动蛋白重链的抗血清被用于分离果蝇cDNA克隆。对其中一个cDNA克隆产生的蛋白质的蛋白水解片段进行免疫印迹分析，结果表明这些cDNA克隆编码果蝇驱动蛋白的重链。最大cDNA的体外合成产物在十二烷基硫酸钠/聚丙烯酰胺凝胶上与果蝇驱动蛋白重链共迁移，并在存在ATP的不可水解类似物5'-腺苷酰亚胺二磷酸的情况下与紫杉醇稳定的微管结合，但在存在ATP或0.1 M KCl的情况下则不结合。对cDNA克隆的分析表明，果蝇中编码驱动蛋白重链的单个基因位于多线染色体位置53A。然而，Southern杂交分析表明果蝇基因组中存在相关序列。
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