高柠檬酸可治愈肺炎克雷伯菌中的NifV-表型。
Homocitrate cures the NifV- phenotype in Klebsiella pneumoniae.
作者信息
Hoover T R, Imperial J, Ludden P W, Shah V K
机构信息
Department of Biochemistry, College of Agriculture and Life Sciences, University of Wisconsin-Madison 53706.
出版信息
J Bacteriol. 1988 Apr;170(4):1978-9. doi: 10.1128/jb.170.4.1978-1979.1988.
Abstract
Dinitrogenase was isolated from a culture of a Klebsiella pneumoniae NifV- strain derepressed for nitrogenase in the presence of homocitrate. The enzyme isolated from this culture was identical to the wild-type dinitrogenase. These data provide in vivo evidence that the absence of homocitrate is responsible for the NifV- phenotype.
摘要
从肺炎克雷伯菌NifV-菌株的培养物中分离出固氮酶，该菌株在高柠檬酸存在下对固氮酶进行了去阻遏。从该培养物中分离出的酶与野生型固氮酶相同。这些数据提供了体内证据，表明缺乏高柠檬酸是导致NifV-表型的原因。
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本文引用的文献
1
Hydrogen evolution and exchange, and conversion of N2O to N2 by soybean root nodules.
Biochim Biophys Acta. 1960 Jan 15;37:273-9. doi: 10.1016/0006-3002(60)90234-1.
2
Requirement of nifV gene for production of wild-type nitrogenase enzyme in Klebsiella pneumoniae.
Nature. 1981 Aug 13;292(5824):655-6. doi: 10.1038/292655a0.
3
Microbial iron compounds.
Annu Rev Biochem. 1981;50:715-31. doi: 10.1146/annurev.bi.50.070181.003435.
4
Role of the nifQ gene product in the incorporation of molybdenum into nitrogenase in Klebsiella pneumoniae.
J Bacteriol. 1984 Apr;158(1):187-94. doi: 10.1128/jb.158.1.187-194.1984.
5
Nitrogenase from nifV mutants of Klebsiella pneumoniae contains an altered form of the iron-molybdenum cofactor.
Biochem J. 1984 Jan 1;217(1):317-21. doi: 10.1042/bj2170317.
6
Isolation of a molybdenum--iron cluster from nitrogenase.
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3438-40. doi: 10.1073/pnas.78.6.3438.
7
The nitrogenase system from Azotobacter: two-enzyme requirement for N2 reduction, ATP-dependent H2 evolution, and ATP hydrolysis.
Proc Natl Acad Sci U S A. 1966 Sep;56(3):979-86. doi: 10.1073/pnas.56.3.979.
8
Nitrogenase. I. Repression and derepression of the iron-molybdenum and iron proteins of nitrogenase in Azotobacter vinelandii.
Biochim Biophys Acta. 1972 Feb 28;256(2):498-511. doi: 10.1016/0005-2728(72)90078-3.
9
The nifH gene product is required for the synthesis or stability of the iron-molybdenum cofactor of nitrogenase from Klebsiella pneumoniae.
Eur J Biochem. 1986 Oct 15;160(2):371-7. doi: 10.1111/j.1432-1033.1986.tb09981.x.
10
Isolation and characterization of nitrogenase from Klebsiella pneumoniae.
Methods Enzymol. 1986;118:511-9. doi: 10.1016/0076-6879(86)18097-9.
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