Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Biotechnology & Biological Science Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Nanomedicine (Lond). 2019 Jul;14(14):1827-1840. doi: 10.2217/nnm-2018-0520. Epub 2019 Jul 5.
We aim to use peptide nucleic acid (PNA) for antisense therapy against bovine viral diarrhea virus (BVDV), a surrogate model of human hepatitis C virus, and introduce an optimal approach for delivering PNA into the cell. PNA was designed for hybridization to the 5'-untranslated region of BVDV RNA in order to form a heteroduplex structure and inhibit the translation and replication of virus. Gold nanoparticles (AuNPs) were used as a delivery system for PNA. The cellular uptake of PNA-AuNPs and inhibition of BVDV infection in the middle stage of viral replication were found. Further research is warranted to develop AuNPs as a potential vehicle for delivering PNA in order to remove viruses from the infected cells.
我们旨在使用肽核酸（PNA）进行反义治疗，针对牛病毒性腹泻病毒（BVDV），这是人类丙型肝炎病毒的替代模型，并引入一种将 PNA 递送入细胞的最佳方法。PNA 的设计用于与 BVDV RNA 的 5'-非翻译区杂交，以形成异源双链结构并抑制病毒的翻译和复制。金纳米粒子（AuNPs）被用作 PNA 的递送系统。发现 PNA-AuNPs 的细胞摄取和病毒复制中期的 BVDV 感染抑制。需要进一步的研究来开发 AuNPs 作为递送 PNA 的潜在载体，以便从感染细胞中去除病毒。
