Nagi Shaymaa M, Hassan Shahinaz N, Abd El-Alim Sameh H, Elmissiry Mostafa M
Assistant Researcher Professor in Restorative and Dental Materials Department, Oral and Dental Research division, National Research Centre, Egypt.
Researcher in Restorative and Dental Materials Department, Oral and Dental Research division, National Research Centre, Egypt.
J Clin Exp Dent. 2019 May 1;11(5):e401-e407. doi: 10.4317/jced.55556. eCollection 2019 May.
Remineralizing of bleached enamel is a common procedure that aims to compensate enamel mineral lose. This study aimed to evaluate the remineralization effectiveness of experimentally prepared grape seed extract hydrogels (GSE) compared to fluoride gel on bleached enamel.
Thirty extracted maxillary incisor were bleached using white smile bleaching agent. Bleached specimens were divided into three groups (10/group) according to the remineralizing agents tested: [GSE 6%, GSE 10%, or fluoride gel]. After bleaching and remineralization procedure, the specimens were stored in artificial saliva at 37°C. Micro-hardness and Energy-Dispersive X-ray and ultra-morphological evaluation were tested at baseline, after bleaching and after remineralization.
Statistical significant decrease on mean micro-hardness values had resulted after bleaching procedure compared to baseline, followed by a significant increase in GSE (10%) and fluoride groups. GSE (6%) application showed the least statistical significant mean micro-hardness, which was statistically insignificant different compared to bleaching procedure. Elemental analysis results revealed a statistical significant decrease on Ca, and Ca/P ratios (At%) values after bleaching compared to baseline, followed by a significant increase after treatment with all tested remineralizing gels. SEM photomicrograph of sound enamel shows smooth uniform appearance with few pores. Bleached enamel showed irregular pitted disorganized enamel surface. Bleached enamel exposed to GSE and fluoride gel showed occlusion of enamel surface porosities and precipitates of different sizes.
The specially prepared GSE hydrogels has positive effects on the remineralization process of bleached enamel making it an effective natural agent with remineralizing potential. Remineralization, bleaching, grape seed extract, fluoride, enamel.
漂白牙釉质再矿化是一种常见的操作,旨在补偿牙釉质矿物质流失。本研究旨在评估实验制备的葡萄籽提取物水凝胶(GSE)与氟凝胶相比对漂白牙釉质的再矿化效果。
使用美白漂白剂对30颗拔除的上颌切牙进行漂白。根据测试的再矿化剂,将漂白后的标本分为三组(每组10个):[6% GSE、10% GSE或氟凝胶]。在漂白和再矿化程序后,将标本储存在37°C的人工唾液中。在基线、漂白后和再矿化后测试显微硬度、能量色散X射线和超微形态学评估。
与基线相比,漂白程序后平均显微硬度值有统计学意义的下降,随后GSE(10%)组和氟组有显著增加。应用6% GSE显示出最小的统计学显著平均显微硬度,与漂白程序相比在统计学上无显著差异。元素分析结果显示,与基线相比,漂白后钙和钙/磷比(原子%)值有统计学意义的下降,随后用所有测试的再矿化凝胶处理后有显著增加。健康牙釉质的扫描电子显微镜照片显示表面光滑均匀,孔隙较少。漂白后的牙釉质显示出不规则的凹坑和无序的牙釉质表面。暴露于GSE和氟凝胶的漂白牙釉质显示牙釉质表面孔隙被封闭,并有不同大小的沉淀物。
特别制备的GSE水凝胶对漂白牙釉质的再矿化过程有积极作用,使其成为一种具有再矿化潜力的有效天然剂。再矿化、漂白、葡萄籽提取物、氟、牙釉质。