天然物质对乳牙人工诱导龋损再矿化潜力的评估：一项研究。

Evaluation of Remineralization Potential of Natural Substances on Artificially Induced Carious Lesions in Primary Teeth: An Study.

作者信息

Ravi Pooja V, Sekar Rajakumar, Ramar Kavitha

机构信息

Department of Pediatric and Preventive Dentistry, SRM Kattankulathur Dental College and Hospital, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India.

出版信息

Int J Clin Pediatr Dent. 2023 Mar-Apr;16(2):244-250. doi: 10.5005/jp-journals-10005-2569.

DOI:10.5005/jp-journals-10005-2569

PMID:37519954

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10373788/

Abstract

AIM

To evaluate the remineralizing potential of natural substances on artificially induced caries lesions in primary teeth.

MATERIALS AND METHODS

A total of 50 primary molar teeth were selected and subjected to a demineralization process. Then samples were randomly divided into five groups for the remineralization process. Group I-colophony, group II-5% sodium fluoride (NaF) + colophony, group III-grape seed extract (GSE) + colophony, group IV-5% NaF + colophony + 10% peptide, and group V- GSE + colophony + 10% peptide. All the groups were subjected to remineralization using a brushing stimulator for 3,000 cycles. Assessment was done using Vickers hardness testing machine for evaluating the enamel surface microhardness (SMH) and scanning electron microscopy-energy dispersive X-ray analysis (SEM-EDX) for evaluating the surface morphology and mineral content, before and after demineralization and after remineralization, the obtained data was analyzed statistically using IBM Statistical Package for the Social Sciences (SPSS) software version 25.

RESULTS

The enamel microhardness results of this study revealed that remineralization of enamel was highest in group V (212.83 ± 64.416) and least in group II (137.83 ± 26.324) -value of 0.038. SEM-EDX analysis revealed high calcium (Ca) and fluoride (F) content in groups II and IV, which was significant (-value of 0.001) from other groups. Surface morphology evaluated with SEM revealed spherical globular agglomerates and scaffolding deposits on the enamel surface in groups III and V resembling the remineralization process.

CONCLUSION

Grape seed extract (GSE) with colophony and peptide is a superior natural alternative to NaF. Colophony also exhibited remineralizing potential in primary enamel.

CLINICAL SIGNIFICANCE

Natural remineralizing agents like GSE, colophony, and its combination serves as a potential alternative to overcome the toxic effect on long-term usage of F. These natural substances can be applicable in clinical conditions by incorporating toothpaste and varnish, which can be used as an alternative or adjuvant to the topical application of F.

HOW TO CITE THIS ARTICLE

Pooja V Ravi, Rajakumar S, Kavitha Ramar. Evaluation of Remineralization Potential of Natural Substances on Artificially Induced Carious Lesions in Primary Teeth: An Study. Int J Clin Pediatr Dent 2023;16(2):244-250.

摘要

目的

评估天然物质对乳牙人工诱导龋损的再矿化潜力。

材料与方法

共选取50颗乳磨牙，进行脱矿处理。然后将样本随机分为五组进行再矿化处理。第一组 - 松香，第二组 - 5%氟化钠（NaF）+松香，第三组 - 葡萄籽提取物（GSE）+松香，第四组 - 5% NaF +松香+ 10%肽，第五组 - GSE +松香+ 10%肽。所有组均使用刷牙模拟器进行3000次循环的再矿化处理。使用维氏硬度测试机评估釉质表面显微硬度（SMH），并使用扫描电子显微镜 - 能量色散X射线分析（SEM - EDX）评估脱矿前、脱矿后和再矿化后的表面形态和矿物质含量。使用IBM社会科学统计软件包（SPSS）25版对获得的数据进行统计学分析。

结果

本研究的釉质显微硬度结果显示，第五组的釉质再矿化程度最高（212.83±64.416），第二组最低（137.83±26.324） - p值为0.038。SEM - EDX分析显示，第二组和第四组的钙（Ca）和氟（F）含量较高，与其他组相比差异显著（p值为0.001）。用SEM评估的表面形态显示，第三组和第五组的釉质表面有球形团聚体和支架状沉积物，类似于再矿化过程。