Blackshaw J K, Fenwick D C, Beattie A W, Allan D J
Department of Animal Sciences and Production, University of Queensland, St Lucia, Australia.
Lab Anim. 1988 Jan;22(1):67-75. doi: 10.1258/002367788780746674.
Euthanasia of chickens, young and mature rats, and mice was assessed using chloroform, carbon dioxide and ether. Behavioural patterns were recorded to give some indication of the stress involved. Carbon dioxide induced collapse faster (11.2 +/- 0.4 s) than chloroform (18.9 +/- 0.4 s) or ether (greater than 60 s). With regard to the time taken to death, in carbon dioxide mice had the shortest time (48 +/- 10 s) and mature rats had the longest time (135 +/- 10 s). In chloroform, the only difference was the delayed onset of death (127 +/- 10 s) in the chicken. Behavioural patterns were similar for the chicken in carbon dioxide and chloroform, except for wing flapping, even when unconscious, in carbon dioxide. Chloroform is recommended as more aesthetically acceptable for euthanasia of chickens. Carbon dioxide is recommended for the euthanasia of both rats and mice, considering behavioural criteria. Ether is unsuitable as a euthanasia method as it is dangerous, slow acting and an irritant.
使用氯仿、二氧化碳和乙醚对鸡、幼年和成年大鼠以及小鼠实施安乐死，并记录行为模式以了解其中涉及的应激情况。二氧化碳导致动物倒下的速度比氯仿（18.9±0.4秒）或乙醚（超过60秒）更快（11.2±0.4秒）。关于致死所需时间，在二氧化碳环境中，小鼠用时最短（48±10秒），成年大鼠用时最长（135±10秒）。在氯仿环境中，唯一的差异是鸡的死亡延迟（127±10秒）。二氧化碳和氯仿环境中鸡的行为模式相似，但在二氧化碳环境中，即使鸡失去意识后仍会扑打翅膀。考虑到美观因素，推荐使用氯仿对鸡实施安乐死。考虑到行为标准，推荐使用二氧化碳对大鼠和小鼠实施安乐死。乙醚不适合作为安乐死方法，因为它危险、起效慢且具有刺激性。
