台湾 Caspase-8 基因型与口腔癌风险的关联。
Association of Caspase-8 Genotypes With Oral Cancer Risk in Taiwan.
机构信息
Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, R.O.C.
Department of Otolaryngology, China Medical University Hospital, Taichung, Taiwan, R.O.C.
出版信息
In Vivo. 2019 Jul-Aug;33(4):1151-1156. doi: 10.21873/invivo.11585.
BACKGROUND/AIM: Recently, mounting evidence has shown that caspase-8 (CASP8) rs3834129 (-652, 6N insertion/deletion) polymorphism may serve as a genetic biomarker for personal risk of various cancer types. The contribution of CASP8 rs3834129 polymorphism has been investigated in several oral cancer populations, but not in Taiwan. This study investigated the role of CASP8 rs3834129 polymorphism on oral risk in Taiwan.
MATERIALS AND METHODS
CASP8 rs3834129 polymorphic genotypes were determined and their associations with oral cancer risk were investigated among 788 patients with oral cancer and 956 age- and gender-matched healthy controls via polymerase chain reaction-restrictive fragment length polymorphism (PCR-RFLP) methodology. In addition, the interaction of CASP8 rs3834129 genotype with personal behavior and clinicopathological features were also examined.
RESULTS
The frequencies of II, ID and DD genotypes for CASP8 rs3834129 were 57.5, 36.5 and 6.0% in the patient group and 54.0, 39.0 and 7.0% in the healthy control group, respectively (p for trend=0.3052), genotypes were not significantly differentially distributed between the two groups. The comparisons in allelic frequency distribution also supported the findings that the D variant allele may not serve as a determinant of risk for oral cancer. There was no interaction of CASP8 rs3834129 genotype with age, gender, smoking, alcohol or betel quid consumption in regard to oral cancer risk.
CONCLUSION
Our results indicate that the caspase-8 genotype does not appear to play a direct role in personal susceptibility to oral cancer in Taiwan.
背景/目的：最近，越来越多的证据表明，半胱氨酸天冬氨酸蛋白酶 8（CASP8）rs3834129（-652，6N 插入/缺失）多态性可能是各种癌症类型个体风险的遗传生物标志物。已经在几个口腔癌人群中研究了 CASP8 rs3834129 多态性的作用，但在台湾没有研究过。本研究调查了 CASP8 rs3834129 多态性在台湾人口腔癌风险中的作用。
材料和方法
通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，确定了 CASP8 rs3834129 多态性基因型，并在 788 例口腔癌患者和 956 例年龄和性别匹配的健康对照者中研究了其与口腔癌风险的关系。此外，还检查了 CASP8 rs3834129 基因型与个人行为和临床病理特征的相互作用。
结果
CASP8 rs3834129 的 II、ID 和 DD 基因型频率分别为患者组的 57.5%、36.5%和 6.0%，健康对照组的 54.0%、39.0%和 7.0%（趋势检验 p=0.3052），两组基因型分布无显著差异。等位基因频率分布的比较也支持了 D 变异等位基因可能不是口腔癌发病风险决定因素的发现。CASP8 rs3834129 基因型与年龄、性别、吸烟、饮酒或嚼槟榔与口腔癌风险之间无相互作用。
结论
我们的结果表明，半胱氨酸天冬氨酸蛋白酶 8 基因型似乎不会直接影响台湾人患口腔癌的易感性。
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