Mohamadnia Abdolreza, Bayat Mohammad, Norouzi Melika, Gharayagh Zandi Zahra, Shirzadian Fereshteh, Kazempoor Dizaji Mehdi, Soltani Mohamad Hosein, Fotook Kiaei Seyedeh Zahra, Varahram Mohammad, Ahmadi Farnaz, Shafaghi Shadi, Bahrami Naghmeh
Chronic Respiratory Diseases Research Center, NRITLD, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
School of Advanced Technologies in Medicine, Department of Biotechnology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Asian Pac J Cancer Prev. 2025 Apr 1;26(4):1225-1231. doi: 10.31557/APJCP.2025.26.4.1225.
Oral cancer is one of the malignant tumors of the head and neck region, which is associated with high mortality rates and has various negative effects on the aesthetics of patients. Therefore, access to high-quality care for early detection and appropriate surgical and drug treatments is crucial. To this end, researchers are investigating the mechanisms of carcinogenesis in cells and identifying the factors that affect it. The aim of this study was to investigate the mechanisms by which oral microbiota contributes to carcinogenesis.
Sixty peripheral blood samples were collected from oral squamous cell carcinoma (OSCC) patients with (30 samples) and without (30 samples) of oral infection, referred to the Cancer Institute of Tehran University of Medical Sciences. Real-time PCR was performed to determine the expression levels of miR-92, miR-26, miR-486, Bak, Bax, and Caspase-8 genes.
MiR-92 and miR-26 relative expression were higher in the OSCC patients with oral infection compared to OSCC patients without oral infection. However, relative expression of miR-486, Bak, Bax, and Caspase-8 was significantly decreased in patients with oral infection compared to OSCC patients without oral infection. Conclusion: The results showed that oral microbiome prevents apoptosis and promotes the development of cancerous tissue in OSCC patients. The identification of a link between oral infection and microRNA and apoptosis-related gene expression could provide researchers with the opportunity to formulate innovative methods for the prevention or management of OSCC.
口腔癌是头颈部恶性肿瘤之一，死亡率高，对患者美观有诸多负面影响。因此，获得高质量的早期检测以及合适的手术和药物治疗至关重要。为此，研究人员正在研究细胞中的致癌机制并确定影响该机制的因素。本研究的目的是探讨口腔微生物群促进致癌作用的机制。
从德黑兰医科大学癌症研究所转诊的患有（30份样本）和未患有（30份样本）口腔感染的口腔鳞状细胞癌（OSCC）患者中采集60份外周血样本。进行实时PCR以确定miR-92、miR-26、miR-486、Bak、Bax和Caspase-8基因的表达水平。
与未患口腔感染的OSCC患者相比，患口腔感染的OSCC患者中miR-92和miR-26的相对表达更高。然而，与未患口腔感染的OSCC患者相比，患口腔感染的患者中miR-486、Bak、Bax和Caspase-8的相对表达显著降低。结论：结果表明，口腔微生物群可防止OSCC患者细胞凋亡并促进癌组织发展。确定口腔感染与微小RNA以及凋亡相关基因表达之间的联系可为研究人员提供制定预防或管理OSCC创新方法的机会。
