基因型与口腔癌风险的关联。

Association of Genotypes With Oral Cancer Risk.

机构信息

Department of Otorhinolaryngology, China Medical University Hospital, Taichung, Taiwan, R.O.C.

Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, R.O.C.

出版信息

In Vivo. 2022 Nov-Dec;36(6):2669-2677. doi: 10.21873/invivo.13002.

DOI:10.21873/invivo.13002

PMID:36309370

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9677796/

Abstract

BACKGROUND/AIM: The over-expression of enhancer of zeste homolog 2 (EZH2) protein is found in oral cancer tissues. However, the genetic role of the enhancer of EZH2 in the etiology of oral cancer is unknown. The aim of this study was to evaluate the association of EZH2 genotypes with oral cancer risk among Taiwanese.

MATERIALS AND METHODS

Three polymorphic variants of EZH2, rs887569 (C to T), rs41277434 (A to C), and rs3757441 (T to C), were analyzed regarding their association with oral cancer risk among 958 oral cancer patients and the same number of healthy controls by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). In addition, the interaction of EZH2 rs887569, rs41277434, and rs3757441 genotypes with personal behaviors such as smoking, alcohol drinking, and betel quid chewing were also examined.

RESULTS

The EZH2 genotypes rs887569, rs41277434, and rs3757441, were not significantly associated with oral cancer risk (p for trend=0.1735, 0.5658, and 0.4606, respectively). The analysis of allelic frequency distribution also supported the findings that the variant alleles at EZH2 rs887569, rs41277434, and rs3757441 may not serve as determinants of oral cancer risk (all p>0.05). There was no interaction between EZH2 rs887569, rs41277434, or rs3757441 genotypes with personal smoking, alcohol drinking or betel quid chewing behaviors.

CONCLUSION

EZH2 genotypes cannot predict oral cancer risk in Taiwan.

摘要

背景/目的：在口腔癌组织中发现增强子的 EZH2 蛋白过表达。然而，EZH2 增强子在口腔癌病因学中的遗传作用尚不清楚。本研究旨在评估 EZH2 基因型与台湾人口腔癌风险的相关性。

材料和方法

通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析 EZH2 的三个多态性变体 rs887569（C 到 T）、rs41277434（A 到 C）和 rs3757441（T 到 C）与 958 例口腔癌患者和相同数量的健康对照者的口腔癌风险之间的关系。此外，还检查了 EZH2 rs887569、rs41277434 和 rs3757441 基因型与个人行为（如吸烟、饮酒和咀嚼槟榔）之间的相互作用。

结果

EZH2 基因型 rs887569、rs41277434 和 rs3757441 与口腔癌风险无显著相关性（趋势检验 p 值分别为 0.1735、0.5658 和 0.4606）。等位基因频率分布的分析也支持了以下发现，即 EZH2 rs887569、rs41277434 和 rs3757441 的变异等位基因可能不是口腔癌风险的决定因素（均 p>0.05）。EZH2 rs887569、rs41277434 或 rs3757441 基因型与个人吸烟、饮酒或咀嚼槟榔行为之间无相互作用。