白藜芦醇抑制肿瘤坏死因子-α诱导的炎症反应，以保护糖尿病大鼠免受肾缺血/再灌注损伤。

Resveratrol inhibits TNF-α-induced inflammation to protect against renal ischemia/reperfusion injury in diabetic rats.

作者信息

Wang Min, Weng Xiaodong, Chen Hui, Chen Zhiyuan, Liu Xiuheng

机构信息

PhD, Department of Urology, Renmin Hospital of Wuhan University, Wuhan, China. Conception and design of the study, acquisition and interpretation of data, manuscript writing.

PhD, Department of Urology, Renmin Hospital of Wuhan University, Wuhan, China. Conception and design of the study; acquisition, analysis and interpretation of data; critical revision.

出版信息

Acta Cir Bras. 2020 Jul 3;35(5):e202000506. doi: 10.1590/s0102-865020200050000006.

PURPOSE

To examine effects of resveratrol on renal ischemia/ reperfusion injury (I/R) in a streptozotocin (STZ)-induced diabetic rat model.

METHODS

Twenty-four male Sprague Dawley rats were treated with STZ injection for the development of diabetes, and divided into the following groups: Sham group, I/R group and Resveratrol group (n=8). Resveratrol (RSV) was administered at a dose of 10 mg.kg-1.d-1 fourteen days prior to suffering from I/R. Renal function, histology, SOD, MDA, TUNEL assay and expression of TNF-α, IL-1β, NF-κB-P65, COX-2 and Caspase3, Bcl2 and Bax were analyzed.

RESULTS

Administration of RSV significantly reduced the serum levels of renal dysfunction and injury markers, including creatinine, blood urea nitrogen and MDA; in the other hand, it significantly increased the serum levels of SOD. The protective effect of RSV was also reflected on histologic evaluation. RSV reduced the number of apoptotic cells as determined by TUNEL assay. RSV significantly reduced the protein expression of TNF-α, IL-1β, NF-κB-P65, COX-2 and Caspase3, and Bax. Meanwhile, RSV significantly increased the protein expression of Bcl2.

CONCLUSION

RSV attenuated I/R-induced renal injury in diabetic rats through the modulation of oxidative stress and TNF-α-stimulated inflammation.

目的

在链脲佐菌素（STZ）诱导的糖尿病大鼠模型中，研究白藜芦醇对肾缺血/再灌注损伤（I/R）的影响。

方法

24只雄性Sprague Dawley大鼠经STZ注射诱导糖尿病，分为以下几组：假手术组、I/R组和白藜芦醇组（n = 8）。在遭受I/R前14天，以10 mg·kg-1·d-1的剂量给予白藜芦醇（RSV）。分析肾功能、组织学、超氧化物歧化酶（SOD）、丙二醛（MDA）、TUNEL检测以及肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、核因子-κB-P65（NF-κB-P65）、环氧化酶-2（COX-2）和半胱天冬酶3（Caspase3）、B细胞淋巴瘤-2（Bcl2）和Bax的表达。

结果

给予RSV显著降低了肾功能不全和损伤标志物的血清水平，包括肌酐、血尿素氮和MDA；另一方面，它显著提高了SOD的血清水平。RSV的保护作用也体现在组织学评估上。通过TUNEL检测确定，RSV减少了凋亡细胞的数量。RSV显著降低了TNF-α、IL-1β、NF-κB-P65、COX-2和Caspase3以及Bax的蛋白表达。同时，RSV显著提高了Bcl2的蛋白表达。