Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment Instrument, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan RD, Shanghai 200240, P.R. China.
National Center for Translational Medicine, Collaborative Innovational Center for System Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.
Theranostics. 2019 May 24;9(12):3443-3458. doi: 10.7150/thno.33266. eCollection 2019.
Recently, there is one-fifth of human deaths caused by cancer, leading to cancer treatment remains a hard nut to crack in the medical field. Therefore, as an emerging diagnostic technology, mesoporous nanomaterials-based drug delivery systems integrated diagnosis and therapy have aroused tremendous interest owing to visually targeting effect and superior therapy efficacy compared with traditional cancer treatment. In this work, we have successfully synthesized mesoporous carbon-gold hybrid nanozyme nanoprobes, whereby mesoporous carbon nanospheres were doped with small gold nanoparticles (OMCAPs) and further stabilized with a complex of reduced serum albumin and folic acid (rBSA-FA). After loading IR780 iodide, the OMCAPs@ rBSA-FA@IR780 nanoprobes were finally accomplished for real-time imaging, photothermal/photodynamic and nanozyme oxidative therapy. Herein, acid oxidized MCAPs possessed large surface area and numerous -COOH groups, which could be used to surface chemically modify numerous targeting molecules and load abundant NIR dye IR780, as well as be acted as photothermal reagents to enhance photothermal therapy effect. In addition, the small Au NPs embedded in OMCAPs were utilized as nanozyme to catalyze HO located in tumor cells to generate ·OH for intracellular oxidative damage of tumor. Besides, as a commonly used near-infrared (NIR) fluorescence dye, the loaded IR780 iodide could not only apply for real-time imaging, but also effectively enhance photo-thermal therapy (PTT) upon the 808 nm laser irradiation. Moreover, FA molecules could enhance the targeted efficiency of the nanoprobes to the gastric tumor site. According to the systematical study and , our fabricated nanoprobes based on carbon-gold hybrid (OMCAPs@ rBSA-FA@IR780) revealed excellent tumor targeting efficacy, long tumor retention and favorably therapeutic effect for tumor. All the results demonstrated that here synthesized probes exhibited excellently diagnostic and therapeutic performance, indicating our technology may potentially offer an outstanding strategy for tumor-targeting theranostics.
最近，有五分之一的人类死亡是由癌症引起的，这导致癌症治疗仍然是医学领域的一个难题。因此，作为一种新兴的诊断技术，介孔纳米材料基药物输送系统集诊断和治疗于一体，由于具有可视化靶向作用和优于传统癌症治疗的疗效，引起了极大的兴趣。在这项工作中，我们成功地合成了介孔碳-金杂化纳米酶探针，其中介孔碳纳米球掺杂了小金纳米粒子（OMCAPs），并进一步用还原血清白蛋白和叶酸（rBSA-FA）的复合物稳定。负载 IR780 碘化物后，最终完成了 OMCAPs@rBSA-FA@IR780 纳米探针的制备，用于实时成像、光热/光动力和纳米酶氧化治疗。在此，酸氧化的 MCAPs 具有较大的表面积和大量的-COOH 基团，可用于表面化学修饰大量靶向分子并负载丰富的近红外染料 IR780，也可用作光热试剂以增强光热治疗效果。此外，嵌入 OMCAPs 中的小 Au NPs 被用作纳米酶，以催化位于肿瘤细胞中的 HO 生成·OH，用于肿瘤细胞内的氧化损伤。此外，作为一种常用的近红外（NIR）荧光染料，负载的 IR780 碘化物不仅可用于实时成像，而且在 808nm 激光照射下还可有效增强光热治疗（PTT）。此外，FA 分子可以增强纳米探针对胃肿瘤部位的靶向效率。根据系统研究[22,23]，我们构建的基于碳金杂化（OMCAPs@rBSA-FA@IR780）的纳米探针表现出优异的肿瘤靶向效果、长肿瘤滞留时间和良好的肿瘤治疗效果。所有结果表明，所合成的探针表现出优异的诊断和治疗性能，表明我们的技术可能为肿瘤靶向治疗提供一种有前途的策略。
