Department of Ultrasound, Qilu Hospital of Shandong University, Jinan 250012, China.
Key Laboratory of Special Functional Aggregated Materials, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China.
Ultrasonics. 2019 Nov;99:105947. doi: 10.1016/j.ultras.2019.105947. Epub 2019 Jul 2.
Nanobubbles (NBs) have received wide attention as theranostic agents and been extensively explored in various applications, especially in cancer. The aim of this study was to develop a novel kind of NBs which possess high echogenicity and good stability. This novel ultrasonic nanobubbles (ST68/PLA-PEG NBs) consist of perfluoropropane gas stabilized by Span 60 and Tween 80 (ST68) surfactant and synthesized PLA-PEG-NH block copolymers, and were prepared through the methods of mechanical shaking and low-speed centrifugation. A series of experiments were carried out to evaluate the physicochemical properties, echogenicity and cytotoxicity of this novel NBs. According to the amount ratio of copolymers to surfactant, the NBs were divided into 5 groups (0%, 5%, 10%, 15% and 20%). Group "10%" were the optimum NBs, with a size of 675.6 nm, polydispersity index of 0.39. Moreover, these NBs gave a maximum contrast intensity of 31.0 ± 0.2 dB over baseline and little loss of contrast signal after 10 min. In conclusion, this novel kind of ST68/PLA-PEG NBs which exhibited a high echogenicity and good stability were successfully prepared, and they may offer a potential strategy for drug delivery and tumor-targeted theranostic.
纳米气泡（NBs）作为治疗诊断试剂受到广泛关注，并在各种应用中得到了广泛的探索，尤其是在癌症领域。本研究旨在开发一种具有高回声性和良好稳定性的新型 NBs。这种新型超声纳米气泡（ST68/PLA-PEG NBs）由 Span 60 和 Tween 80（ST68）表面活性剂稳定的全氟丙烷气体以及合成的 PLA-PEG-NH 嵌段共聚物组成，并通过机械搅拌和低速离心的方法制备。进行了一系列实验来评估这种新型 NBs 的理化性质、回声性和细胞毒性。根据共聚物与表面活性剂的比例，将 NBs 分为 5 组（0%、5%、10%、15%和 20%）。“10%”组为最佳 NBs，粒径为 675.6nm，多分散指数为 0.39。此外，这些 NBs 在基础线上产生最大的对比强度为 31.0±0.2dB，且在 10min 后对比信号几乎没有损失。总之，成功制备了具有高回声性和良好稳定性的新型 ST68/PLA-PEG NBs，为药物输送和肿瘤靶向治疗提供了一种潜在的策略。
