Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Horticulture, Hainan University, Haikou 570228, China.
Int J Mol Sci. 2019 Jul 6;20(13):3321. doi: 10.3390/ijms20133321.
Radish is a crucial vegetable crop of the family with many varieties and large cultivated area in China. Radish is a cool season crop, and there are only a few heat tolerant radish varieties in practical production with little information concerning the related genes in response to heat stress. In this work, some physiological parameter changes of young leaves under short-term heat stress were detected. Furthermore, we acquired 1802 differentially expressed mRNAs (including encoding some heat shock proteins, heat shock factor and heat shock-related transcription factors), 169 differentially expressed lncRNAs and three differentially expressed circRNAs (novel_circ_0000265, novel_circ_0000325 and novel_circ_0000315) through strand-specific RNA sequencing technology. We also found 10 differentially expressed miRNAs (ath-miR159b-3p, athmiR159c, ath-miR398a-3p, athmiR398b-3p, ath-miR165a-5p, ath-miR169g-3p, novel_86, novel_107, novel_21 and ath-miR171b-3p) by small RNA sequencing technology. Through function prediction and enrichment analysis, our results suggested that the significantly possible pathways/complexes related to heat stress in radish leaves were circadian rhythm-plant, photosynthesis-antenna proteins, photosynthesis, carbon fixation in photosynthetic organisms, arginine and proline metabolism, oxidative phosphorylation, peroxisome and plant hormone signal transduction. Besides, we identified one lncRNA-miRNA-mRNAs combination responsive to heat stress. These results will be helpful for further illustration of molecular regulation networks of how radish responds to heat stress.
萝卜是十字花科的一种重要蔬菜作物，在中国有许多品种和大面积种植。萝卜是一种冷季作物，在实际生产中只有少数耐热萝卜品种，关于其对热胁迫的相关基因的信息很少。在这项工作中，检测了短期热胁迫下幼叶的一些生理参数变化。此外，我们通过链特异性 RNA 测序技术获得了 1802 个差异表达的 mRNAs（包括编码一些热休克蛋白、热休克因子和热休克相关转录因子）、169 个差异表达的 lncRNAs 和三个差异表达的 circRNAs（novel_circ_0000265、novel_circ_0000325 和 novel_circ_0000315）。我们还通过小 RNA 测序技术发现了 10 个差异表达的 miRNAs（ath-miR159b-3p、athmiR159c、ath-miR398a-3p、ath-miR398b-3p、ath-miR165a-5p、ath-miR169g-3p、novel_86、novel_107、novel_21 和 ath-miR171b-3p）。通过功能预测和富集分析，我们的结果表明，与萝卜叶片热胁迫显著相关的可能途径/复合物是昼夜节律-植物、光合作用-天线蛋白、光合作用、光合生物中的碳固定、精氨酸和脯氨酸代谢、氧化磷酸化、过氧化物酶体和植物激素信号转导。此外，我们鉴定了一个响应热胁迫的 lncRNA-miRNA-mRNAs 组合。这些结果将有助于进一步阐明萝卜响应热胁迫的分子调控网络。
