Department of Agricultural Sciences, University of Naples Federico II, Portici, Naples, Italy.
School of Science, Engineering & Technology, Division of Food & Drink, University of Abertay, Dundee, Scotland, UK.
Phytochem Anal. 2019 Sep;30(5):556-563. doi: 10.1002/pca.2850. Epub 2019 Jul 8.
Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is one of the most devastating and harmful pests of tomato (Solanum lycopersicum) crops causing up to 80-100% yield losses. A large arsenal of plant metabolites is induced by the leafminer feeding including defence compounds that could differ among varieties.
To compare the metabolomic changes of different genotypes of tomato (tolerant "T", susceptible "S" and "F1" hybrid obtained between T and S) after exposition to T. absoluta.
Nuclear magnetic resonance (NMR) spectroscopy followed by multivariate data analysis were performed to analyse the metabolic profiles of control and infested samples on three different tomato genotypes.
Signals related to GABA (γ-aminobutyric acid) were relatively much higher in all infested samples compared to the non-infested plants used as control. Infested T genotype samples were the most abundant in organic acids, including fatty acids and acyl sugars, chlorogenic acid, neo-chlorogenic acid and feruloyl quinic acid, indicating a clear link between the exposure to leafminer. Results also showed an increase of trigonelline in all tomato varieties after exposition to T. absoluta.
Metabolomics approach based on NMR spectroscopy followed by multivariate data analysis allowed for a detailed metabolite profile of plant defences, providing fundamental information for breeding programmes in plant crops.
烟粉虱（Meyrick）（鳞翅目：草螟科）是番茄（Solanum lycopersicum）作物最具破坏性和危害性的害虫之一，可导致高达 80-100%的产量损失。叶虫取食会诱导大量植物代谢物产生，包括不同品种之间可能存在差异的防御化合物。
比较不同基因型番茄（抗虫“T”、感虫“S”和 T 和 S 杂交获得的“F1”杂种）暴露于烟粉虱后的代谢组变化。
采用核磁共振（NMR）光谱法结合多元数据分析，分析了三种不同番茄基因型对照和受侵染样品的代谢谱。
与用作对照的未受侵染植物相比，所有受侵染样品中的 GABA（γ-氨基丁酸）相关信号相对较高。受侵染的 T 基因型样品中含有丰富的有机酸，包括脂肪酸和酰基糖、绿原酸、新绿原酸和阿魏酰奎宁酸，表明与叶虫暴露之间存在明显联系。结果还表明，所有番茄品种在暴露于烟粉虱后，三羟吡啶的含量增加。
基于 NMR 光谱的代谢组学方法结合多元数据分析，可以对植物防御的详细代谢物图谱进行分析，为植物作物的育种计划提供了基础信息。
