College of Pharmacy, Woosuk University, Jeonju, 55338, Republic of Korea.
College of Materials and Chemistry Engineering, Tongren University, Guizhou, 554300, China.
Arch Pharm Res. 2017 Jul;40(7):825-835. doi: 10.1007/s12272-017-0920-3. Epub 2017 Jun 30.
This study contributes to the continual discovery of lifespan-extending compounds from plants, using the Caenorhabditis elegans model system. An ethyl acetate soluble fraction of methanol extract from the heartwood of Caesalpinia sappan showed a significant lifespan-extending activity. Subsequent activity-guided chromatography of the ethyl acetate-soluble fraction led to the isolation of brazilin. Brazilin showed potent 2,2-diphenyl-1-picrylhydrazyl radical scavenging and superoxide anion quenching activities and also revealed a lifespan-extending activity in C. elegans under normal culture conditions. Brazilin also exhibited the protective effects against thermal, oxidative and osmotic stress conditions to improve the survival rate of the nematode. Furthermore, brazilin elevated superoxide dismutase (SOD) activity and decreased intracellular reactive oxygen species accumulation in C. elegans. Further studies showed that brazilin-mediated increased stress tolerance of worms could be due to increased expressions of stress resistance proteins such as heat shock protein (HSP-16.2) and superoxide dismutase (SOD-3). Besides, there were no significant, brazilin-induced changes in aging-related factors, including progeny production, food intake, and growth, indicating brazilin influences longevity activity independent of affecting these factors. Brazilin increased the body movement of aged worms, indicating brazilin affects the healthspan and lifespan of nematode. These results suggest that brazilin contributes to the lifespan of C. elegans under both normal and stress conditions by increasing the expressions of stress resistance proteins.
本研究利用秀丽隐杆线虫模型系统，为从植物中不断发现延长寿命的化合物做出了贡献。甲醇提取物的心材乙酸乙酯可溶部分显示出显著的延长寿命活性。随后对乙酸乙酯可溶部分进行活性导向色谱分离，得到了巴西苏木素。巴西苏木素具有很强的 2,2-二苯基-1-苦基肼基自由基清除和超氧阴离子猝灭活性，并且在正常培养条件下也显示出延长秀丽隐杆线虫寿命的活性。巴西苏木素还表现出对热、氧化和渗透胁迫条件的保护作用，提高了线虫的存活率。此外，巴西苏木素可提高秀丽隐杆线虫中超氧化物歧化酶（SOD）的活性并减少细胞内活性氧的积累。进一步的研究表明，巴西苏木素介导的线虫对胁迫的耐受性增加可能是由于应激抵抗蛋白如热休克蛋白（HSP-16.2）和超氧化物歧化酶（SOD-3）的表达增加所致。此外，巴西苏木素没有引起与衰老相关的因素（包括后代产生、食物摄入和生长）的显著变化，这表明巴西苏木素通过影响这些因素来影响寿命活性。巴西苏木素增加了年老线虫的身体运动，表明巴西苏木素影响线虫的健康寿命和寿命。这些结果表明，巴西苏木素通过增加应激抵抗蛋白的表达，在正常和应激条件下延长秀丽隐杆线虫的寿命。
