Peng Lijun, Li Shaobin, Li Yuchan, Wan Minghui, Fang Xisheng, Zhao Yongxin, Zuo Wei, Long De, Xuan Yiwen
Department of Thoracic Surgery, Guangzhou General Hospital of The People's Liberation Army, Guangzhou, Guangdong 510010, P.R. China.
Department of Cardiothoracic Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong 510280, P.R. China.
Oncol Lett. 2019 Jul;18(1):137-144. doi: 10.3892/ol.2019.10333. Epub 2019 May 7.
The present study aimed to evaluate microRNA- 20b-5p (miR-20b-5p) expression in non-small cell lung cancer (NSCLC), and investigate the effects of miR-20b-5p expression on NSCLC cell proliferation and migration. Reverse transcription-quantitative polymerase chain reaction was performed to measure the expression level of miR-20b-5p in NSCLC tissues and cell lines. Cell Counting Kit-8 and wound healing assays were used to measure cell proliferation and migration. A dual-luciferase reporter assay was performed to validate B-cell translocation gene 3 (BTG3) as a target of miR-20b-5p. It was identified that the expression level of miR-20b-5p is elevated in NSCLC tissues and cell lines. miR-20b-5p overexpression was revealed to promote NSCLC cell proliferation and migration. Furthermore, BTG3 was identified as a direct target of miR-20b-5p, and BTG3 overexpression reversed a miR-20b-5p mimic-induced increase in cell proliferation and migration. In summary, the present study revealed that miR-20b-5p promotes NSCLC cell proliferation and migration by targeting BTG3, which may assist with the development of a novel therapeutic target for the treatment of NSCLC.
本研究旨在评估非小细胞肺癌（NSCLC）中微小RNA-20b-5p（miR-20b-5p）的表达，并探讨miR-20b-5p表达对NSCLC细胞增殖和迁移的影响。采用逆转录-定量聚合酶链反应检测NSCLC组织和细胞系中miR-20b-5p的表达水平。使用细胞计数试剂盒-8和伤口愈合试验检测细胞增殖和迁移。进行双荧光素酶报告基因检测以验证B细胞易位基因3（BTG3）是miR-20b-5p的靶标。结果发现，miR-20b-5p在NSCLC组织和细胞系中的表达水平升高。miR-20b-5p过表达可促进NSCLC细胞增殖和迁移。此外，BTG3被确定为miR-20b-5p的直接靶标，BTG3过表达可逆转miR-20b-5p模拟物诱导的细胞增殖和迁移增加。总之，本研究表明miR-20b-5p通过靶向BTG3促进NSCLC细胞增殖和迁移，这可能有助于开发治疗NSCLC的新型治疗靶点。
