Biswas T, Miller F W, Takagaki Y, Plotz P H
J Immunol Methods. 1987 Apr 16;98(2):243-8. doi: 10.1016/0022-1759(87)90011-1.
We have developed an enzyme-linked immunosorbent assay (ELISA) specific for autoantibodies directed against the autoantigen Jo-1 (histidyl-tRNA synthetase) using antigen prepared biochemically from HeLa cells. No other patient sera, including those containing antibodies directed at threonyl-tRNA synthetase or alanyl-tRNA synthetase, reacted in the assay. Screening of sera from 169 patients with a variety of autoimmune and neuromuscular diseases confirmed that anti-Jo-1 antibodies are confined to a subgroup of patients with pure polymyositis, pure dermatomyositis, or myositis associated with another rheumatic disease.
我们利用从HeLa细胞生化制备的抗原，开发了一种针对抗自身抗原Jo-1（组氨酰-tRNA合成酶）自身抗体的酶联免疫吸附测定（ELISA）。在该测定中，没有其他患者血清发生反应，包括那些含有抗苏氨酰-tRNA合成酶或丙氨酰-tRNA合成酶抗体的血清。对169例患有各种自身免疫性和神经肌肉疾病患者的血清进行筛查，证实抗Jo-1抗体仅限于患有单纯性多发性肌炎、单纯性皮肌炎或与另一种风湿性疾病相关的肌炎的患者亚组。
