一种通过 mRNA 展示体外选择订书肽抑制剂的新策略。
A new strategy for the in vitro selection of stapled peptide inhibitors by mRNA display.
机构信息
Department of Chemistry, Virginia Commonwealth University, 1001 West Main Street, P. O. Box 842006, Richmond, VA 23284, USA.
School of Mathematics & Natural Sciences, Chemistry & Biochemistry, 118 College Drive #5043, Hattiesburg, MS 39406, USA.
出版信息
Chem Commun (Camb). 2019 Aug 7;55(61):8959-8962. doi: 10.1039/c8cc10192b. Epub 2019 Jul 10.
Abstract
Hydrocarbon stapled peptides are promising therapeutics for inhibition of intracellular protein-protein interactions. Here we develop a new high-throughput strategy for hydrocarbon stapled peptide discovery based on mRNA display of peptides containing α-methyl cysteine and cyclized with m-dibromoxylene. We focus on development of a peptide binder to the HPV16 E2 protein.
摘要
碳氢化合物订书肽是抑制细胞内蛋白质-蛋白质相互作用的有前途的治疗方法。在这里，我们开发了一种基于含有α-甲基半胱氨酸的肽的 mRNA 展示和与 m-二溴对二甲苯环化的新型高通量碳氢化合物订书肽发现策略。我们专注于开发针对 HPV16 E2 蛋白的肽结合物。
相似文献
1
A new strategy for the in vitro selection of stapled peptide inhibitors by mRNA display.
Chem Commun (Camb). 2019 Aug 7;55(61):8959-8962. doi: 10.1039/c8cc10192b. Epub 2019 Jul 10.
2
Peptide and peptide library cyclization via bromomethylbenzene derivatives.
Methods Mol Biol. 2015;1248:105-17. doi: 10.1007/978-1-4939-2020-4_8.
3
Structure-based derivation and optimization of YAP-like coactivator-derived peptides to selectively target TEAD family transcription factors by hydrocarbon stapling and cyclization.
Chem Biol Drug Des. 2021 Jun;97(6):1129-1136. doi: 10.1111/cbdd.13813. Epub 2021 Mar 23.
4
Four-base codon mediated mRNA display to construct peptide libraries that contain multiple nonnatural amino acids.
Nucleic Acids Res. 2006 Jan 5;34(1):e7. doi: 10.1093/nar/gnj003.
5
Selection of Peptides and Proteins-Advantages of mRNA Display.
ACS Synth Biol. 2020 Feb 21;9(2):181-190. doi: 10.1021/acssynbio.9b00419. Epub 2020 Jan 14.
6
In vitro selection of protein and peptide libraries using mRNA display.
Methods Mol Biol. 2009;535:293-314. doi: 10.1007/978-1-59745-557-2_17.
7
Peptides inhibitory for the transcriptional regulatory function of human papillomavirus E2.
Clin Cancer Res. 2003 Nov 1;9(14):5423-8.
8
Phage display methods for selecting zinc finger proteins with novel DNA-binding specificities.
Methods Enzymol. 1996;267:129-49. doi: 10.1016/s0076-6879(96)67010-4.
9
Selection of Peptide-Bismuth Bicycles Using Phage Display.
ACS Chem Biol. 2024 May 17;19(5):1040-1044. doi: 10.1021/acschembio.4c00099. Epub 2024 Apr 15.
10
Selective thioether macrocyclization of peptides having the N-terminal 2-chloroacetyl group and competing two or three cysteine residues in translation.
Org Biomol Chem. 2012 Aug 14;10(30):5783-6. doi: 10.1039/c2ob25306b. Epub 2012 Mar 14.
引用本文的文献
1
Tackling Undruggable Targets with Designer Peptidomimetics and Synthetic Biologics.
Chem Rev. 2024 Nov 27;124(22):13020-13093. doi: 10.1021/acs.chemrev.4c00423. Epub 2024 Nov 14.
2
A recombinant approach for stapled peptide discovery yields inhibitors of the RAD51 recombinase.
Chem Sci. 2023 Nov 21;14(47):13915-13923. doi: 10.1039/d3sc03331g. eCollection 2023 Dec 6.
3
Using display technologies to identify macrocyclic peptide antibiotics.
Biochim Biophys Acta Mol Cell Res. 2023 Jun;1870(5):119473. doi: 10.1016/j.bbamcr.2023.119473. Epub 2023 Apr 1.
4
Development of a Cyclic, Cell Penetrating Peptide Compatible with In Vitro Selection Strategies.
ACS Chem Biol. 2023 Apr 21;18(4):746-755. doi: 10.1021/acschembio.2c00680. Epub 2023 Mar 15.
5
Enzymatic Macrolactamization of mRNA Display Libraries for Inhibitor Selection.
ACS Chem Biol. 2023 Jan 20;18(1):166-175. doi: 10.1021/acschembio.2c00828. Epub 2022 Dec 9.
6
Hyperaccurate Ribosomes for Improved Genetic Code Reprogramming.
ACS Synth Biol. 2022 Jun 17;11(6):2193-2201. doi: 10.1021/acssynbio.2c00150. Epub 2022 May 12.
7
Non-canonical Amino Acid Substrates of E. coli Aminoacyl-tRNA Synthetases.
Chembiochem. 2022 Jan 5;23(1):e202100299. doi: 10.1002/cbic.202100299. Epub 2021 Sep 22.
8
Directing evolution of novel ligands by mRNA display.
Chem Soc Rev. 2021 Aug 21;50(16):9055-9103. doi: 10.1039/d1cs00160d. Epub 2021 Jun 24.
9
Small and Simple, yet Sturdy: Conformationally Constrained Peptides with Remarkable Properties.
Int J Mol Sci. 2021 Feb 5;22(4):1611. doi: 10.3390/ijms22041611.
10
Expanding the Chemical Diversity of Genetically Encoded Libraries.
ACS Comb Sci. 2020 Dec 14;22(12):712-733. doi: 10.1021/acscombsci.0c00179. Epub 2020 Nov 9.
本文引用的文献
1
Constrained peptides' time to shine?
Nat Rev Drug Discov. 2018 Jul 30;17(8):531-533. doi: 10.1038/nrd.2018.125.
2
Stapling of unprotected helical peptides photo-induced intramolecular thiol-yne hydrothiolation.
Chem Sci. 2016 May 1;7(5):3325-3330. doi: 10.1039/c6sc00106h. Epub 2016 Feb 5.
3
Importance of Net Hydrophobicity in the Cellular Uptake of All-Hydrocarbon Stapled Peptides.
Mol Pharm. 2018 Mar 5;15(3):1332-1340. doi: 10.1021/acs.molpharmaceut.7b01130. Epub 2018 Feb 21.
4
Ribosomal incorporation of backbone modified amino acids via an editing-deficient aminoacyl-tRNA synthetase.
Org Biomol Chem. 2018 Feb 14;16(7):1073-1078. doi: 10.1039/c7ob02931d.
5
Hydrocarbon constrained peptides - understanding preorganisation and binding affinity.
Chem Sci. 2016 Jun 1;7(6):3694-3702. doi: 10.1039/c5sc04048e. Epub 2016 Feb 29.
6
Effect of Stapling Architecture on Physiochemical Properties and Cell Permeability of Stapled α-Helical Peptides: A Comparative Study.
Chembiochem. 2017 Nov 2;18(21):2087-2093. doi: 10.1002/cbic.201700352. Epub 2017 Sep 26.
7
Enhancing Specific Disruption of Intracellular Protein Complexes by Hydrocarbon Stapled Peptides Using Lipid Based Delivery.
Sci Rep. 2017 May 11;7(1):1763. doi: 10.1038/s41598-017-01712-5.
8
Highly Constrained Bicyclic Scaffolds for the Discovery of Protease-Stable Peptides via mRNA Display.
ACS Chem Biol. 2017 Mar 17;12(3):795-804. doi: 10.1021/acschembio.6b01006. Epub 2017 Feb 1.
9
Biophysical determinants for cellular uptake of hydrocarbon-stapled peptide helices.
Nat Chem Biol. 2016 Oct;12(10):845-52. doi: 10.1038/nchembio.2153. Epub 2016 Aug 22.
10
Review stapling peptides using cysteine crosslinking.
Biopolymers. 2016 Nov;106(6):843-852. doi: 10.1002/bip.22877.
Zotero 插件