基于连接酶放大反应的单个量子点纳米传感器的组装用于碱性磷酸酶的灵敏检测。
Ligase amplification reaction-catalyzed assembly of a single quantum dot-based nanosensor for sensitive detection of alkaline phosphatase.
机构信息
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China.
出版信息
Chem Commun (Camb). 2019 Aug 7;55(61):8963-8966. doi: 10.1039/c9cc04369a. Epub 2019 Jul 10.
We develop a simple method for sensitive detection of alkaline phosphatase (ALP) based on the ligase amplification reaction-catalyzed assembly of a single quantum dot (QD)-based nanosensor. This nanosensor requires only a single ligase enzyme to achieve ultrahigh sensitivity with a detection limit of 5.63 × 10 U mL, and can be applied for kinetic analysis, inhibitor screening, and ALP measurement in cell extracts.
我们开发了一种简单的方法，用于基于连接酶扩增反应催化组装基于单个量子点(QD)的纳米传感器灵敏检测碱性磷酸酶(ALP)。该纳米传感器仅需单种连接酶即可实现超高灵敏度，检测限低至 5.63×10 U mL，并可用于动力学分析、抑制剂筛选以及细胞提取物中的 ALP 测量。
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