水肉桂提取物（ACE-c）从肉桂树皮造成细胞凋亡的人宫颈癌细胞株（SiHa）通过丧失线粒体膜电位。
Aqueous cinnamon extract (ACE-c) from the bark of Cinnamomum cassia causes apoptosis in human cervical cancer cell line (SiHa) through loss of mitochondrial membrane potential.
机构信息
Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth University Medical College Campus, Pune, Maharashtra, India.
出版信息
BMC Cancer. 2010 May 18;10:210. doi: 10.1186/1471-2407-10-210.
BACKGROUND
Chemoprevention, which includes the use of synthetic or natural agents (alone or in combination) to block the development of cancer in human beings, is an extremely promising strategy for cancer prevention. Cinnamon is one of the most widely used herbal medicines with diverse biological activities including anti-tumor activity. In the present study, we have reported the anti-neoplastic activity of cinnamon in cervical cancer cell line, SiHa.
METHODS
The aqueous cinnamon extract (ACE-c) was analyzed for its cinnamaldehyde content by HPTLC analysis. The polyphenol content of ACE-c was measured by Folin-Ciocalteau method. Cytotoxicity analysis was performed by MTT assay. We studied the effect of cinnamon on growth kinetics by performing growth curve, colony formation and soft agar assays. The cells treated with ACE-c were analyzed for wound healing assay as well as for matrix metalloproteinase-2 (MMP-2) expression at mRNA and protein level by RT-PCR and zymography, respectively. Her-2 protein expression was analyzed in the control and ACE-c treated samples by immunoblotting as well as confocal microscopy. Apoptosis studies and calcium signaling assays were analyzed by FACS. Loss of mitochondrial membrane potential (Deltapsim) in cinnamon treated cells was studied by JC-1 staining and analyzed by confocal microscopy as well as FACS.
RESULTS
Cinnamon alters the growth kinetics of SiHa cells in a dose-dependent manner. Cells treated with ACE-c exhibited reduced number of colonies compared to the control cells. The treated cells exhibited reduced migration potential that could be explained due to downregulation of MMP-2 expression. Interestingly, the expression of Her-2 oncoprotein was significantly reduced in the presence of ACE-c. Cinnamon extract induced apoptosis in the cervical cancer cells through increase in intracellular calcium signaling as well as loss of mitochondrial membrane potential.
CONCLUSION
Cinnamon could be used as a potent chemopreventive drug in cervical cancer.
背景
化学预防包括使用合成或天然药物（单独或联合使用）来阻止人类癌症的发展，是一种极具前景的癌症预防策略。肉桂是最广泛使用的草药之一，具有多种生物活性，包括抗肿瘤活性。在本研究中，我们报道了肉桂在宫颈癌细胞系 SiHa 中的抗肿瘤活性。
方法
通过 HPTLC 分析测定水提肉桂提取物（ACE-c）中的肉桂醛含量。采用 Folin-Ciocalteau 法测定 ACE-c 的多酚含量。通过 MTT 分析进行细胞毒性分析。通过生长曲线、集落形成和软琼脂测定研究肉桂对生长动力学的影响。用 ACE-c 处理的细胞进行划痕愈合试验以及通过 RT-PCR 和凝胶电泳分别在 mRNA 和蛋白水平上研究基质金属蛋白酶-2（MMP-2）的表达。通过免疫印迹和共聚焦显微镜分析 Her-2 蛋白在对照和 ACE-c 处理样品中的表达。通过 FACS 分析凋亡研究和钙信号转导。用 JC-1 染色研究肉桂处理细胞中线粒体膜电位（Deltapsim）的丧失，并通过共聚焦显微镜和 FACS 进行分析。
结果
肉桂以剂量依赖的方式改变 SiHa 细胞的生长动力学。与对照细胞相比，用 ACE-c 处理的细胞中集落数量减少。处理后的细胞迁移潜力降低，这可以解释为 MMP-2 表达下调。有趣的是，在存在 ACE-c 的情况下，Her-2 癌蛋白的表达明显降低。肉桂提取物通过增加细胞内钙信号和线粒体膜电位丧失诱导宫颈癌细胞凋亡。
结论
肉桂可作为宫颈癌的有效化学预防药物。
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