School of Nuclear Science and Engineering, Oregon State University, Corvallis, OR, USA.
Department of Radiation Oncology, Washington University School of Medicine, St. Louis, MO, USA.
Int J Radiat Biol. 2019 Nov;95(11):1484-1497. doi: 10.1080/09553002.2019.1642537. Epub 2019 Jul 25.
The aim of this study is to build a computational model to investigate the cell dose and cell DNA damage distribution of a multicellular tissue system under the irradiation. In this work, we developed a computational model for quantifying cell dose and double strand break (DSB) number in a multicellular system by simulating the radiation transport in 2D and 3D cell culture. The model was based on an open-source radiation transport package, Geant4 with Geant4-DNA physics. First, the computational multicellular system was created using a developed program, CelllMaker. Second, the radiation transport simulation for cells was conducted using Geant4 package with the Geant4-DNA physics to obtain the cellular dose and cellular DSB yield. Using the method described in this work, it is possible to obtain the cellular dose and DNA damage simultaneously. The developed model provides a solution for quantifying the cellular dose and cellular DNA damage which are not easily determined in a radiobiological experiment. With limited validation data for the model, this preliminary study provides a roadmap for building a comprehensive toolkit for simulating cellular dose and DNA damage of multicellular tissue systems.
本研究旨在构建一个计算模型，以研究照射下多细胞组织系统的细胞剂量和细胞 DNA 损伤分布。在这项工作中，我们通过模拟二维和三维细胞培养中的辐射传输，开发了一种用于量化多细胞系统中细胞剂量和双链断裂 (DSB) 数量的计算模型。该模型基于开源辐射传输包 Geant4 和 Geant4-DNA 物理。首先，使用开发的程序 CelllMaker 创建计算多细胞系统。其次，使用 Geant4 包和 Geant4-DNA 物理进行细胞辐射传输模拟，以获得细胞剂量和细胞 DSB 产额。使用本工作中描述的方法，可以同时获得细胞剂量和 DNA 损伤。开发的模型提供了一种量化细胞剂量和细胞 DNA 损伤的解决方案，而这些在放射生物学实验中不容易确定。由于模型的验证数据有限，本初步研究为构建模拟多细胞组织系统细胞剂量和 DNA 损伤的综合工具包提供了路线图。
