Wang C Y, Huang H Y, Kuo K L, Hsieh Y Z
Department of Applied Chemistry, National Chiao Tung University, Hsinchu, Taiwan, R.O.C.
J Chromatogr A. 1998 Apr 3;802(1):225-31. doi: 10.1016/s0021-9673(97)01070-4.
This study presents a high-performance capillary electrophoresis (CE) method to analyze five constituents of Puerariae radix, an important crude herb used in Chinese medicine. Puerarin, daidzin, daidzein, genistein and biochanin A are the bioactive constituents of Puerariae radix. Herein, those analytes were successfully separated within 6 min using a pH 10.1 borax-NaOH buffer. The effects of pH value and concentration of the running buffer on the separation of the five analytes were also examined. The relative standard deviations of the analytes' migration times were less than 0.38% under the optimized separation conditions. Notably, the correlation coefficients of the analytes' linear calibration graphs exceeded 0.998. Moreover, the amounts of the five constituents in three different Puerariae radix samples were determined by the CE method with a relatively simple extraction procedure.
