在 BacT/Alert® Virtuo 血培养系统中培养假体周围组织可提高并加快假体关节感染的检测速度。
Culturing periprosthetic tissue in BacT/Alert® Virtuo blood culture system leads to improved and faster detection of prosthetic joint infections.
机构信息
Research Group for Host-Microbe Interactions, Department of Medical Biology, Faculty of Health Sciences, UiT, The Arctic University of Norway, Tromsø, Norway.
Department of Microbiology and Infection Control, University Hospital of North Norway, Tromsø, Norway.
出版信息
BMC Infect Dis. 2019 Jul 10;19(1):607. doi: 10.1186/s12879-019-4206-x.
BACKGROUND
Blood culture bottles (BCBs) provide a semiautomated method for culturing periprosthetic tissue specimens. A study evaluating BCBs for culturing clinical samples other than body fluids is needed before implementation into clinical practice. Our objective was to evaluate use of the BacT/Alert® Virtuo blood culture system for culturing periprosthetic tissue specimens.
METHODS
The study was performed through the analysis of spiked (n = 36) and clinical (n = 158) periprosthetic tissue samples. Clinical samples were analyzed by the BCB method and the results were compared to the conventional microbiological culture-based method for time to detection and microorganisms identified.
RESULTS
The BacT/Alert® Virtuo blood culture system detected relevant bacteria for prosthetic joint infection in both spiked and clinical samples. The BCB method was found to be as sensitive (79%) as the conventional method (76%) (p = 0.844) during the analyses of clinical samples. The BCB method yielded positive results much faster than the conventional method: 89% against 27% detection within 24 h, respectively. The median detection time was 11.1 h for the BCB method (12 h and 11 h for the aerobic and the anaerobic BCBs, correspondingly).
CONCLUSION
We recommend using the BacT/Alert® Virtuo blood culture system for analyzing prosthetic joint tissue, since this detect efficiently and more rapidly a wider range of bacteria than the conventional microbiological method.
背景
血培养瓶（BCB）为培养假体周围组织标本提供了一种半自动化的方法。在将其应用于临床实践之前，需要对其用于培养除体液以外的临床样本的性能进行评估。我们的目的是评估 BacT/Alert® Virtuo 血培养系统用于培养假体周围组织标本的效果。
方法
本研究通过分析人工污染（n=36）和临床（n=158）假体周围组织样本进行。临床样本采用 BCB 法进行分析，并将结果与传统的基于微生物培养的方法进行比较，以评估检测时间和鉴定的微生物。
结果
BacT/Alert® Virtuo 血培养系统在人工污染和临床样本中均能检测到与假体关节感染相关的细菌。在对临床样本的分析中，BCB 法与传统方法的灵敏度相当（79%比 76%，p=0.844）。BCB 法比传统方法更快地产生阳性结果：分别为 89%和 27%在 24 小时内检测到，中位检测时间分别为 11.1 小时和 11 小时（需氧和厌氧 BCB 分别为 12 小时和 11 小时）。
结论
我们推荐使用 BacT/Alert® Virtuo 血培养系统分析假体关节组织，因为与传统的微生物学方法相比，它能更高效、更快地检测到更广泛的细菌。
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