Department of Emergency Medicine, Institute of Health Sciences, Gyeongsang National University College of Medicine and Gyeongsang National University Changwon Hospital, Changwon, Korea.
Department of Laboratory Medicine, Institute of Health Sciences, Gyeongsang National University College of Medicine and Gyeongsang National University Changwon Hospital, Changwon, Korea.
Ann Lab Med. 2019 May;39(3):278-283. doi: 10.3343/alm.2019.39.3.278.
BacT/Alert Virtuo (BioMérieux, Durham, NC, USA) is a recently developed blood culture system that includes functions of automatic registration, loading, and unloading of the blood culture bottles, as well as measurement of blood volume. We compared the performances between the BacT/Alert Virtuo and 3D (BioMérieux) blood culture systems.
A total of 952 patients (1,904 sets) visiting an university-affiliated hospital in Korea for blood cultures were enrolled. Five milliliters of blood was added into each of the two aerobic (FA Plus) and two anaerobic (FN Plus) bottles of the Virtuo and 3D systems for a single set. Positive rate and time to detection (TTD) were compared between the two systems.
The positive rates were 8.3% and 8.4% in FA Plus bottles and 7.8% and 8.3% in FN Plus bottles, in the Virtuo and 3D systems, respectively (>0.05). Median TTDs were shorter in the Virtuo than in the 3D system for all isolates (11.5 hours [N=305] vs 11.8 hours [N=318], <0.001), Staphylococcus aureus (N=38; 14.3 hours vs 16.0 hours, =0.021), and Escherichia coli (N=117; 10.4 hours vs 11.0 hours, <0.001).
The Virtuo has the potential to detect pathogens early in all bottle types. This might improve the prognosis of sepsis by allowing for implementation of expeditious management.
BacT/Alert Virtuo（生物梅里埃，美国北卡罗来纳州达勒姆）是一种新开发的血液培养系统，具有自动注册、加载和卸载血培养瓶以及测量血量的功能。我们比较了 BacT/Alert Virtuo 和 3D（生物梅里埃）血培养系统的性能。
共纳入韩国一家大学附属医院的 952 名（1904 套）接受血培养的患者。每个 Virtuo 和 3D 系统的两个需氧瓶（FA Plus）和两个厌氧瓶（FN Plus）各加入 5 毫升血，作为一套。比较两种系统的阳性率和检测时间（TTD）。
Virtuo 和 3D 系统 FA Plus 瓶的阳性率分别为 8.3%和 8.4%（>0.05），FN Plus 瓶的阳性率分别为 7.8%和 8.3%。所有分离株的 Virtuo 系统 TTD 中位数均短于 3D 系统（11.5 小时[N=305]与 11.8 小时[N=318]，<0.001），金黄色葡萄球菌（N=38；14.3 小时与 16.0 小时，=0.021）和大肠埃希菌（N=117；10.4 小时与 11.0 小时，<0.001）。
Virtuo 有可能在所有瓶型中早期检测到病原体。这可能通过实施迅速的管理来改善脓毒症的预后。
