Division of Microbiology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego, 802-786, Warsaw, Poland.
BMC Vet Res. 2019 Jul 10;15(1):238. doi: 10.1186/s12917-019-1973-y.
Mupirocin is one of the few antimicrobials active against methicillin-resistant Staphylococcus aureus (MRSA), and is frequently used for the eradication of MRSA nasal colonisation in humans. Initially, mupirocin resistance was recognised in human S. aureus, including MRSA isolates, then also among coagulase-negative staphylococci (CoNS). Nowadays, mupirocin resistance is occasionally observed in canine staphylococci, along with Staphylococcus pseudintermedius (MRSP) strains, as well as CoNS, which usually show methicillin resistance. In the current study, high-level mupirocin resistance in methicillin-resistant staphylococci isolated from diseased dogs and cats was investigated.
Among 140 methicillin-resistant staphylococci isolates from dogs and cats, three showed high-level mupirocin resistance in a screening test using the agar disk diffusion method. One was recognised as methicillin-resistant S. aureus, one as methicillin-resistant S. pseudintermedius, and one as methicillin-resistant Staphylococcus haemolyticus. S. pseudintermedius and S. aureus were isolated from dogs, S. haemolyticus was obtained from a cat. All isolates showed high-level mupirocin resistance, confirmed by minimum inhibitory concentration (MIC) values of above 1024 μg/ml and the presence of the plasmid-located gene ileS2. This is the first report on the detection of high-level mupirocin resistance (HLMR) in S. haemolyticus of feline origin.
This study revealed the occurrence of HLMR in three Staphylococcus isolates obtained from companion animals in Poland. The results of this study indicate that the monitoring of mupirocin resistance in staphylococci of animal origin, especially in methicillin-resistant isolates, is strongly recommended.
莫匹罗星是少数几种对耐甲氧西林金黄色葡萄球菌 (MRSA) 有效的抗生素之一，常用于消除人类 MRSA 鼻腔定植。最初，人类金黄色葡萄球菌（包括 MRSA 分离株）以及凝固酶阴性葡萄球菌（CoNS）中发现了莫匹罗星耐药性。如今，犬葡萄球菌中偶尔会观察到莫匹罗星耐药性，同时还有耐甲氧西林金黄色葡萄球菌（MRSP）菌株以及通常表现出耐甲氧西林的 CoNS。在本研究中，研究了从患病犬和猫分离的耐甲氧西林葡萄球菌中高水平莫匹罗星耐药性。
在 140 株来自犬和猫的耐甲氧西林葡萄球菌分离株中，有 3 株在琼脂扩散法筛选试验中表现出高水平莫匹罗星耐药性。其中 1 株被鉴定为耐甲氧西林金黄色葡萄球菌，1 株为耐甲氧西林中间葡萄球菌，1 株为耐甲氧西林溶血葡萄球菌。中间葡萄球菌和金黄色葡萄球菌是从犬中分离出来的，而溶血葡萄球菌是从猫中分离出来的。所有分离株均表现出高水平的莫匹罗星耐药性，最低抑菌浓度（MIC）值超过 1024μg/ml 且存在质粒定位基因 ileS2 证实了这一点。这是首次报道在猫源溶血葡萄球菌中检测到高水平莫匹罗星耐药性（HLMR）。
本研究揭示了波兰从伴侣动物中分离到的 3 株葡萄球菌中存在 HLMR。本研究结果表明，强烈建议监测动物源葡萄球菌（特别是耐甲氧西林的分离株）中的莫匹罗星耐药性。
