从有眼分泌物的犬猫中分离的耐甲氧西林金黄色葡萄球菌和耐甲氧西林中间葡萄球菌的流行率和克隆多样性。
Prevalence and clonal diversity of methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius isolated from dogs and cats with eye discharge.
机构信息
1Department of Microbiology, Faculty of Veterinary Medicine, Ataturk University, 25240, Erzurum, Turkey.
2Department of Surgery, Faculty of Veterinary Medicine, Ataturk University, 25240, Erzurum, Turkey.
出版信息
Acta Microbiol Immunol Hung. 2023 Feb 1;70(2):134-141. doi: 10.1556/030.2023.01899. Print 2023 Jun 16.
OBJECTIVES
Methicillin-resistant Staphylococcus (MRS) has originated, spread extensively, and become a prominent source of bacterial infections in both human and animal.
METHODS
We report the prevalence, genetic diversity, and antimicrobial resistance pattern of Staphylococcus pseudintermedius and Staphylococcus aureus strains isolated from dogs and cats with eye discharges.
RESULTS
A total of 12 (6.0%) coagulase-positives staphylococci were identified as (6/200, 3%) S. aureus and (6/200, 3%) S. pseudintermedius. The phenotypic methicillin resistance of S. aureus and S. pseudintermedius were 50.0% (3/6) and 16.7% (1/6), respectively. None of the isolates showed biofilm formation in the microtiter plate assay. The highest resistance (50.0%) for S. pseudintermedius strains was detected against clindamycin and tetracycline. 67.0% of S. aureus isolates were resistant to penicillin-G. The PCR analysis conducted for detection of mecA gene indicated that only one S. aureus isolated from a cat was mecA gene positive. Phylogenetic analysis based on repetitive sequence-based PCR (rep-PCR) showed that all strains were typable and generated PCR products ranging from 800 bp to 4,400 bp. The lineages ST241 and the novel ST2361 in multi-locus sequence typing (MLST) analysis were detected in one methicillin-susceptible S. pseudintermedius and methicillin-resistant S. pseudintermedius of dogs, respectively. In addition, the lineages ST4155 and ST7217 of two methicillin-resistant S. aureus strains of cats were connected epidemiologically to previously reported cases.
CONCLUSIONS
These results indicate epidemiologically related strains (ST241, ST4155, and ST7217) transferring between animals and humans. Therefore, the strategies to combat the widespread MRS should be based on collaboration between human and veterinary medicine under the One Health concept.
目的
耐甲氧西林金黄色葡萄球菌（MRS）已经产生、广泛传播，并成为人类和动物细菌感染的主要来源。
方法
我们报告了从患有眼分泌物的狗和猫中分离出的中间葡萄球菌和金黄色葡萄球菌菌株的流行情况、遗传多样性和抗菌药物耐药模式。
结果
共鉴定出 12 株凝固酶阳性葡萄球菌（6.0%），其中 6 株（3%）为金黄色葡萄球菌，6 株（3%）为中间葡萄球菌。金黄色葡萄球菌和中间葡萄球菌的表型耐甲氧西林率分别为 50.0%（3/6）和 16.7%（1/6）。微量滴定板试验中无任何分离株显示生物膜形成。中间葡萄球菌菌株对克林霉素和四环素的耐药率最高（50.0%）。67.0%的金黄色葡萄球菌分离株对青霉素 G 耐药。mecA 基因检测的 PCR 分析表明，只有一只来自猫的金黄色葡萄球菌分离株 mecA 基因阳性。基于重复序列 PCR（rep-PCR）的系统发育分析表明，所有菌株均可分型，产生的 PCR 产物范围为 800 bp 至 4400 bp。多位点序列分型（MLST）分析中检测到 ST241 谱系和新的 ST2361 谱系存在于一只耐甲氧西林敏感的中间葡萄球菌和一只耐甲氧西林的中间葡萄球菌中。此外，两只猫的耐甲氧西林金黄色葡萄球菌分离株的谱系 ST4155 和 ST7217 在流行病学上与以前报道的病例有关。
结论
这些结果表明，动物和人类之间存在具有流行病学相关性的菌株（ST241、ST4155 和 ST7217）转移。因此，应该在“同一健康”概念下，基于人类医学和兽医医学的合作，制定对抗广泛传播的耐甲氧西林金黄色葡萄球菌的策略。
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