Department of Traditional Chinese Medicine, Hebei North University, Zhangjiakou 075000, Hebei Province, China.
Department of Gastroenterology, People's Hospital of Changshou District, Chongqing 401220, China.
World J Gastroenterol. 2019 Jun 28;25(24):3056-3068. doi: 10.3748/wjg.v25.i24.3056.
Non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disease worldwide. However, to date, there is no ideal therapy for this disease.
To study the effects of Si-Ni-San freeze-dried powder on high fat diet-induced NAFLD in mice.
Twenty-four male C57BL/6 mice were randomized into three groups of eight. The control group (CON) was allowed access to a normal chow diet. The high fat diet group (FAT) and Si-Ni-San group (SNS) were allowed access to a high fat diet. The SNS group was intragastrically administered Si-Ni-San freeze-dried powder (5.0 g/kg) once daily, and the CON and FAT groups were intragastrically administered distilled water. After 12 wk, body weight, liver index, visceral fat index, serum alanine aminotransferase (ALT), portal lipopoly-saccharide (LPS), liver tumor necrosis factor (TNF)-α and liver triglycerides were measured. Intestinal microbiota were analyzed using a 16S r DNA sequencing technique.
Compared with the FAT group, the SNS group exhibited decreased body weight, liver index, visceral fat index, serum ALT, portal LPS, liver TNF-α and liver triglycerides ( < 0.05). Intestinal microbiota analysis showed that the SNS group had different bacterial composition and function compared with the FAT group. In particular, genus was a bacterial biomarker of SNS group samples.
The beneficial effects of Si-Ni-San freeze-dried powder on high fat diet-induced NAFLD in mice may be associated with its anti-inflammatory and changing intestinal microbiota effects.
非酒精性脂肪性肝病（NAFLD）是一种常见的慢性肝脏疾病，在全球范围内都很普遍。然而，迄今为止，针对这种疾病还没有理想的治疗方法。
研究思尼散冻干粉对高脂饮食诱导的小鼠非酒精性脂肪性肝病的影响。
将 24 只雄性 C57BL/6 小鼠随机分为三组，每组 8 只。对照组（CON）给予普通饲料。高脂饮食组（FAT）和思尼散组（SNS）给予高脂饮食。SNS 组每日给予思尼散冻干粉（5.0 g/kg）灌胃，CON 和 FAT 组给予蒸馏水灌胃。12 周后，测量体重、肝指数、内脏脂肪指数、血清丙氨酸氨基转移酶（ALT）、门脉脂多糖（LPS）、肝肿瘤坏死因子（TNF）-α和肝甘油三酯。采用 16S rDNA 测序技术分析肠道微生物群。
与 FAT 组相比，SNS 组的体重、肝指数、内脏脂肪指数、血清 ALT、门脉 LPS、肝 TNF-α和肝甘油三酯降低（<0.05）。肠道微生物群分析显示，SNS 组与 FAT 组的细菌组成和功能存在差异。特别是，属是 SNS 组样本的细菌生物标志物。
思尼散冻干粉对高脂饮食诱导的小鼠非酒精性脂肪性肝病的有益作用可能与其抗炎和改变肠道微生物群的作用有关。
