Gene Targets in Ocular Pathogenic for Mitigation of Biofilm Formation to Overcome Antibiotic Resistance.

The present work is an attempt to establish the functionality of genes involved in biofilm formation and antibiotic resistance in an ocular strain of (L-1216/2010) which was isolated and characterized from the Vitreous fluid of a patient with Endophthalmitis. For this purpose, seven separate gene-specific knockout mutants were generated by homologous recombination in ocular . The genes that were mutated included three transmembrane genes (ABC transporter ATP-binding protein), (multidrug efflux system) and (inner membrane protein), coding for non-coding RNA and three metabolic genes (3-3-hydroxyphenylpropionate 1,2-dioxygenase), (2,3-di hydroxyphenylpropionate 1,2-dioxygenase), and (regulatory gene of ). Mutants were validated by sequencing and Reverse transcription-PCR and monitored for biofilm formation by XTT method and confocal microscopy. The antibiotic susceptibility of the mutants was also ascertained. The results indicated that biofilm formation was inhibited in five mutants (Δ, Δ, Δ, Δ, and Δ) and the thickness of biofilm reduced from 17.2 μm in the wildtype to 1.5 to 4.8 μm in the mutants. Mutants Δ and Δ retained the potential to form biofilm. Complementation of the mutants with the wild type gene restored biofilm formation potential in all mutants except in Δ. The 5 mutants which lost their ability to form biofilm (Δ, Δ, Δ, Δ, and Δ) did not exhibit any change in their susceptibility to Ceftazidime, Cefuroxime, Ciprofloxacin, Gentamicin, Cefotaxime, Sulfamethoxazole, Imipenem, Erythromycin, and Streptomycin in the planktonic phase compared to wild type ocular . But Δ was the only mutant with altered MIC to Sulfamethoxazole, Imipenem, Erythromycin, and Streptomycin both in the planktonic and biofilm phase. This is the first report demonstrating the involvement of the metabolic genes and and (regulatory gene of ) in biofilm formation in ocular . In addition we provide evidence that and are required for biofilm formation while and are not required. Mitigation of biofilm formation to overcome antibiotic resistance could be achieved by targeting the genes , and .