Gong Yujie, Yang Hua, Wang Xin, Xia Wenrui, Lv Wentao, Xiao Yingping, Zou Xiaoting
Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Science, Zhejiang University, Hangzhou, China.
Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Front Microbiol. 2019 Jun 25;10:1422. doi: 10.3389/fmicb.2019.01422. eCollection 2019.
The aim of this study was to investigate the effect of fermentation broth from broiler cecal content on the colonization and development of the gut microbiota in newly hatched broiler chicks. The fermentation broth was made by a chemostat system using the cecal content from a donor chicken as the source of inoculum. A total of 120 newly hatched broiler chicks were randomly divided into two groups. One group (F group) was orally inoculated with the fermentation broth, and the other (C group) was treated with an equal amount of sterile PBS solution. 16S rRNA gene sequencing was used to investigate the differences in the cecal microbiota of the broiler chickens between the two groups on days 1, 3, 7, 14, and 28. Moreover, the concentrations of short-chain fatty acids (SCFAs) in the cecal contents were analyzed by gas chromatography. The results showed that the abundances of genera and decreased sharply in the F group on days 1 and 3 by the early intervention with cecal fermentation broth. In contrast, the relative abundance of the genus on days 1, 3, and 7, and the family Ruminococcaceae on days 1, 3, and 28 increased in the F group, respectively. In terms of SCFAs, the concentrations of acetate on day 28, propionic acid on days 1, 3, 7, 14, and 28, butyrate on day 1, and isovalerate on day 14 were significantly higher in the F group compared with the C group. Overall, these results suggest that early intervention with cecal fermentation broth could have beneficial effects on broilers gut health, which might be attributed to the alterations in the gut microbial composition and the increased concentrations of SCFAs.
本研究的目的是探讨肉鸡盲肠内容物发酵液对新孵化肉鸡雏鸡肠道微生物群定殖和发育的影响。发酵液由恒化器系统制备，使用供体鸡的盲肠内容物作为接种物来源。总共120只新孵化的肉鸡雏鸡被随机分为两组。一组（F组）口服接种发酵液，另一组（C组）用等量的无菌PBS溶液处理。使用16S rRNA基因测序研究两组肉鸡在第1、3、7、14和28天盲肠微生物群的差异。此外，通过气相色谱分析盲肠内容物中短链脂肪酸（SCFA）的浓度。结果表明，通过盲肠发酵液的早期干预，F组在第1天和第3天属和属的丰度急剧下降。相反，F组在第1、3和7天属的相对丰度，以及在第1、3和28天瘤胃球菌科的相对丰度分别增加。在SCFA方面，与C组相比，F组在第28天的乙酸盐浓度、在第1、3、7、14和28天的丙酸盐浓度、在第1天的丁酸盐浓度以及在第14天的异戊酸盐浓度显著更高。总体而言，这些结果表明，盲肠发酵液的早期干预可能对肉鸡肠道健康有有益影响，这可能归因于肠道微生物组成的改变和SCFA浓度的增加。
