College of Life Science, Anhui Agricultural University, 230036, Hefei, Anhui, People's Republic of China.
Fisugarpeptide Biology Engineering Co. Ltd., Anhui 237000, People's Republic of China.
J Anim Sci. 2024 Jan 3;102. doi: 10.1093/jas/skae131.
Cecal microbiota has emerged as a prominent intervention target for improving the production and welfare of poultry. This is essential for the overall health and performance of broiler chickens. The current study focused on investigating the effect of cecal microbiota transplantation (CMT) from healthy donor chickens on the growth performance, immunity, and microbial composition of newly hatched chicks and evaluated the effect of sample storage on the microbial diversity of the cecal samples. A healthy "Wannan Yellow Chicken line" was selected as the donor, and 180 1-d-old chicks from the same line were used as recipients for a 60-d feed trial. The chicks were randomly allocated to three groups (60 birds per group) with three replicates in each group. The three treatment groups were CMT-0 (control, normal saline solution), CMT-I (1:12 cecal content, normal saline supplemented with 10% glycerol), and CMT-II (1:6 cecal content, normal saline supplemented with 10% glycerol). The results of weight gain and absolute organ weight showed significant improvements in the CMT-II group compared with the CMT-0 group. Serum IgG level was significantly improved (P < 0.05) in CMT-I compared with that in the CMT-0. However, IL-6 levels increased in CMT-I and then significantly decreased in CMT-II. The cecal microbial diversity of CMT treatment was compared between two groups, fresh samples (FS) and stored samples at-80 °C (SS). The results showed that beneficial taxa, such as Firmicutes and Verrucomicrobiota, were substantially more abundant in both CMT-I and CMT-II than in CMT-0 in both FS and SS. Microbial function analysis at levels 1, 2, and 3 showed improved metabolism, genetic information processing, cellular processes, environmental information processing, and organismal systems in CMT-I and CMT-II for both FS and SS groups. However, the SS group showed decreased microbial diversity and function. To conclude, cecal microbiota transplantation is a promising strategy for enhancing the productivity and health of broiler chickens.
盲肠微生物群已成为提高家禽生产和福利的重要干预靶点。这对于肉鸡的整体健康和性能至关重要。本研究重点研究了来自健康供体鸡的盲肠微生物群移植（CMT）对刚孵化小鸡的生长性能、免疫力和微生物组成的影响，并评估了样品储存对盲肠样品微生物多样性的影响。选择健康的“皖南黄鸡系”作为供体，将同一系的 180 只 1 日龄小鸡作为受体进行 60 天的饲料试验。小鸡被随机分配到三个组（每组 60 只），每组三个重复。三个处理组分别为 CMT-0（对照，生理盐水溶液）、CMT-I（1:12 盲肠内容物，生理盐水补充 10%甘油）和 CMT-II（1:6 盲肠内容物，生理盐水补充 10%甘油）。体重增加和绝对器官重量的结果表明，CMT-II 组与 CMT-0 组相比有显著改善。与 CMT-0 相比，CMT-I 组血清 IgG 水平显著提高（P<0.05）。然而，CMT-I 组的 IL-6 水平升高，然后在 CMT-II 组中显著降低。比较了 CMT 处理的盲肠微生物多样性在两组之间，新鲜样本（FS）和在-80°C 下储存的样本（SS）。结果表明，在 FS 和 SS 组中，CMT-I 和 CMT-II 中的有益类群（如厚壁菌门和疣微菌门）比 CMT-0 显著更多。在 1、2 和 3 级水平上的微生物功能分析表明，FS 和 SS 组的 CMT-I 和 CMT-II 中代谢、遗传信息处理、细胞过程、环境信息处理和生物体系统得到改善。然而，SS 组的微生物多样性和功能下降。总之，盲肠微生物群移植是提高肉鸡生产力和健康水平的一种很有前途的策略。
