Wright M D, Wood P R, Coia G, Cheers C
Department of Microbiology, University of Melbourne, Parkville, Victoria, Australia.
Immunol Cell Biol. 1987 Dec;65 ( Pt 6):505-10. doi: 10.1038/icb.1987.59.
Macrophages were pulsed with Listeria monocytogenes antigens by intraperitoneal injection prior to harvesting and thoroughly washing the cells. The pulsed macrophages were injected into the feet of Listeria-immune or naive mice to elicit a delayed hypersensitivity reaction. Where soluble Listeria antigen was used, presentation by donor macrophages to host T cells required identity within the I region of the H-2 complex. However, presentation of alcohol-killed Listeria organisms or of a living, temperature-sensitive mutant of Listeria was apparently not H-2 restricted. When T cells enriched in vitro for Listeria reactivity were injected into the feet of naive mice, they reacted in an H-2 restricted manner to antigen presented to them either by the pulsed macrophages or host cells apparently acquiring antigen from the original pulsed macrophages.
在收获和彻底洗涤细胞之前，通过腹腔注射用单核细胞增生李斯特菌抗原对巨噬细胞进行脉冲处理。将经脉冲处理的巨噬细胞注射到对李斯特菌免疫或未免疫的小鼠足部，以引发迟发型超敏反应。当使用可溶性李斯特菌抗原时，供体巨噬细胞向宿主T细胞的呈递需要H-2复合体I区内的一致性。然而，用酒精杀死的李斯特菌或李斯特菌的活的温度敏感突变体的呈递显然不受H-2限制。当体外富集对李斯特菌有反应性的T细胞注射到未免疫小鼠的足部时，它们以H-2限制的方式对经脉冲处理的巨噬细胞或显然从原始经脉冲处理的巨噬细胞获得抗原的宿主细胞呈递给它们的抗原产生反应。
