Engineering and Processing Research Division, Malaysian Palm Oil Board, Kajang, Selangor, Malaysia.
J Sep Sci. 2020 Jan;43(1):285-291. doi: 10.1002/jssc.201900342. Epub 2019 Jul 25.
Tocochromanols consisting of tocopherols and tocotrienols, is collectively known as vitamin E. Similarity in their structures, physical and chemical properties rendered the tocochromanols to be subject of chromatography interest. Supercritical fluid chromatography is a highly efficient tool for the separation and analysis of tocochromanols. Separation and analysis of tocochromanols using supercritical fluid chromatography had been carried out in the past using capillary or packed columns. Each of these techniques offer their own advantages and drawbacks. Besides being used for analysis, packed column supercritical fluid chromatography found applications as a purification and content enrichment tool. Emergence of new equipment and stationary phase technologies in recent years also helped in making supercritical fluid chromatography a highly efficient tool for the separation and analysis of tocochromanols. This paper gives an insight into the use of capillary and packed columns in supercritical fluid chromatography for the separation and/or analysis of tocochromanols. The types of stationary phase used, as well as chromatographic conditions are also discussed.
生育三烯酚和生育酚统称为生育酚，统称为维生素 E。由于它们的结构、物理和化学性质相似，生育三烯酚成为色谱法感兴趣的对象。超临界流体色谱是分离和分析生育三烯酚的高效工具。过去，使用毛细管或填充柱进行了超临界流体色谱法分离和分析生育三烯酚。这些技术中的每一种都有其自身的优点和缺点。除了用于分析之外，填充柱超临界流体色谱还被用作纯化和含量富集工具。近年来，新设备和固定相技术的出现也有助于使超临界流体色谱成为分离和分析生育三烯酚的高效工具。本文深入探讨了在超临界流体色谱中使用毛细管柱和填充柱分离和/或分析生育三烯酚的情况。还讨论了所使用的固定相类型以及色谱条件。
