Plachká Kateřina, Pilařová Veronika, Horáček Ondřej, Gazárková Taťána, Vlčková Hana Kočová, Kučera Radim, Nováková Lucie
Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Hradec Králové, Czech Republic.
Department of Pharmaceutical Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy in Hradec Králové, Charles University, Hradec Králové, Czech Republic.
J Sep Sci. 2023 Sep;46(18):e2300431. doi: 10.1002/jssc.202300431. Epub 2023 Aug 11.
Within this review, we thoroughly explored supercritical fluid chromatography (SFC) columns used across > 3000 papers published from the first study carried out under SFC conditions in 1962 to the end of 2022. We focused on the open tubular capillary, packed capillary, and packed columns, their chemistries, dimensions, and trends in used stationary phases with correlation to their specific interactions, advantages, drawbacks, used instrumentation, and application field. Since the 1990s, packed columns with liquid chromatography and SFC-dedicated stationary phases for chiral and achiral separation are predominantly used. These stationary phases are based on silica support modified with a wide range of chemical moieties. Moreover, numerous unconventional stationary phases were evaluated, including porous graphitic carbon, titania, zirconia, alumina, liquid crystals, and ionic liquids. The applications of unconventional stationary phases are described in detail as they bring essential findings required for further development of the supercritical fluid chromatography technique.
在本综述中，我们全面探讨了超临界流体色谱（SFC）柱，这些柱子应用于从1962年首次在SFC条件下开展研究至2022年底发表的3000多篇论文中。我们重点关注了开管毛细管柱、填充毛细管柱和填充柱，它们的化学性质、尺寸以及所用固定相的趋势，并关联了其特定相互作用、优缺点、所用仪器和应用领域。自20世纪90年代以来，主要使用具有用于手性和非手性分离的液相色谱和SFC专用固定相的填充柱。这些固定相基于用多种化学基团改性的硅胶载体。此外，还评估了许多非常规固定相，包括多孔石墨化碳、二氧化钛、氧化锆、氧化铝、液晶和离子液体。非常规固定相的应用被详细描述，因为它们带来了超临界流体色谱技术进一步发展所需的重要发现。
