US Department of Agriculture, Animal and Plant Health Inspection Service, National Wildlife Research Center, Hawaii Field Station, PO Box 10880, Hilo, Hawaii 96721, USA.
Department of Pharmaceutical Science, Daniel K. Inouye College of Pharmacy, University of Hawaii at Hilo, 200 W Kawili Street, Hilo, Hawaii 96720, USA.
J Wildl Dis. 2020 Jan;56(1):203-207. Epub 2019 Jul 11.
The rat lungworm () has emerged as an important human and animal health concern in Hawaii, US. Although the life cycle of the parasite requires both rat and gastropod hosts, other animals acting as paratenic hosts, such as frogs and centipedes, have been identified as sources of infection. We investigated the occurrence of rat lungworm infections in potential paratenic hosts in Hawaii to provide information on how they might be involved in transmission of angiostrongyliasis. We confirmed the presence of rat lungworm in 87% (21/24) of introduced Puerto Rican coqui frogs () in Hilo, Hawaii, by real-time PCR. Additionally, four Cuban greenhouse frogs (), two cane toads (), and three centipedes () were found to be infected. In the frogs and toads, multiple tissue types were positive, including stomach and intestine, muscle, liver, heart, and brain, indicating larval migration. We identified rat lungworm infections in frogs, toads, and centipedes in Hawaii and highlighted the lack of knowledge of the role paratenic hosts may be playing in the transmission and life cycle maintenance of rat lungworm in Hawaii.
美国夏威夷的肺吸虫已成为人类和动物健康的重要关注点。尽管寄生虫的生命周期需要老鼠和腹足纲动物作为宿主，但其他作为副宿主的动物，如青蛙和蜈蚣，已被确定为感染源。我们调查了夏威夷潜在副宿主中肺吸虫感染的发生情况，以提供有关它们如何参与血管圆线虫病传播的信息。我们通过实时 PCR 证实，在夏威夷希洛的 24 只引进的波多黎各库奎蛙中有 87%（21/24）携带肺吸虫。此外，还发现四只古巴温室蛙、两只蔗蟾和三只蜈蚣受到感染。在青蛙和蟾蜍中，多个组织类型呈阳性，包括胃和肠、肌肉、肝脏、心脏和大脑，表明幼虫迁移。我们在夏威夷的青蛙、蟾蜍和蜈蚣中发现了肺吸虫感染，并强调了对副宿主在传播和维持肺吸虫生命周期中可能发挥作用的认识不足。
