比较枯草芽孢杆菌中用于重组酶表达的基因操作方法：复制型还是整合型（CRISPR-Cas9）质粒？
Comparing methods of genetic manipulation in Bacillus subtilis for expression of recombinant enzyme: Replicative or integrative (CRISPR-Cas9) plasmid?
作者信息
Santos Kamila Oliveira, Costa-Filho João, Spagnol Kérolin Luana, Marins Luis Fernando
机构信息
Laboratory of Molecular Biology, Institute of Biological Sciences (ICB), Federal University of Rio Grande (FURG), Av. Itália, Km 8, 96203-900 Rio Grande, RS, Brazil.
Laboratory of Molecular Biology, Institute of Biological Sciences (ICB), Federal University of Rio Grande (FURG), Av. Itália, Km 8, 96203-900 Rio Grande, RS, Brazil.
出版信息
J Microbiol Methods. 2019 Sep;164:105667. doi: 10.1016/j.mimet.2019.105667. Epub 2019 Jul 8.
The present study evaluated the stability of Bacillus subtilis strains transformed with a replicative or integrative plasmid (via CRISPR-Cas9) to express a recombinant phytase. Both transformation methods did not affect the growth of B. subtilis, but the stability of the construct and the enzymatic activity was reduced in the strain transformed with the replicative plasmid.
本研究评估了用复制型或整合型质粒（通过CRISPR-Cas9）转化以表达重组植酸酶的枯草芽孢杆菌菌株的稳定性。两种转化方法均不影响枯草芽孢杆菌的生长，但用复制型质粒转化的菌株中构建体的稳定性和酶活性降低。
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