Zhang Zhen, Yang Junjie, Feng Qiang, Chen Bin, Li Meihui, Liang Cheng, Li Mingyu, Li Zhihui, Xu Qin, Zhang Lei, Chen Wantao
Department of Oral Maxillofacial Head and Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, Shanghai, China.
Front Microbiol. 2019 Jun 26;10:1439. doi: 10.3389/fmicb.2019.01439. eCollection 2019.
Oral squamous cell carcinoma (OSCC) is affected by the interaction between oral pathogen and holobionts, or the combination of the host and its microbial communities. Studies have indicated the structure and feature of the microbiome in OSCC tissue and saliva, the relationships between microbiota and OSCC sites, stages remain unclear. In the present study, OSCC tissue (T), saliva (S) and mouthwash (W) samples were collected from the same subjects and carried out the microbiome study by 16S sequencing. The results showed the T group was significantly different from the S and W groups with the character of lower richness and diversity. Proteobacteria were most enriched in the T group at the phylum level, while Firmicutes were predominant in groups S and W. At the genus level, the predominant taxa of group T were and , and for group S and W, the predominant taxa were and . The genera related to late stage tumors were and , suggesting microbiota may be implicated in OSCC developing. Both compositional and functional analyses indicated that microbes in tumor tissue were potential indicator for the initiation and development of OSCC.
口腔鳞状细胞癌（OSCC）受口腔病原体与微生物群（即宿主与其微生物群落的组合）之间相互作用的影响。研究表明了OSCC组织和唾液中微生物组的结构和特征，但微生物群与OSCC部位、分期之间的关系仍不清楚。在本研究中，从同一受试者收集OSCC组织（T）、唾液（S）和漱口水（W）样本，并通过16S测序进行微生物组研究。结果显示，T组与S组和W组有显著差异，其特征是丰度和多样性较低。在门水平上，变形菌门在T组中最丰富，而厚壁菌门在S组和W组中占主导地位。在属水平上，T组的主要分类群是 和 ，而S组和W组的主要分类群是 和 。与晚期肿瘤相关的属是 和 ，表明微生物群可能与OSCC的发展有关。组成和功能分析均表明，肿瘤组织中的微生物是OSCC发生和发展的潜在指标。
