Hashimoto Kengo, Shimizu Dai, Ueda Sei, Miyabe Satoru, Oh-Iwa Ichiro, Nagao Toru, Shimozato Kazuo, Nomoto Shuji
Department of Oral and Maxillofacial Surgery, Aichi-Gakuin University School of Dentistry, Nagoya, Japan.
Department of Surgery, Aichi-Gakuin University School of Dentistry, Nagoya, Japan.
J Oral Microbiol. 2022 Aug 7;14(1):2105574. doi: 10.1080/20002297.2022.2105574. eCollection 2022.
Recently, the possibility that oral microbiomes is associated with oral squamous cell carcinoma (OSCC) initiation and progression has attracted attention; however, this association is still unclear. Here, we comprehensively analyze the microbiome profiles of saliva samples using next-generation sequencing followed by determining the association between oral microbiome profiles and OSCC.
Microbiome profiles in saliva samples from patients with OSCC, oral leukoplakia (OLK), and postoperative OSCC (Post) were analyzed. Candidate OSCC-associated bacteria were identified by comparing the bacterial diversity and relative abundance of each group based on these microbiome profiles, and their applicability as OSCC detection tools were evaluated.
There were significant differences in genus abundances (, and ) among the groups from saliva samples. In the OSCC group, compared with the OLK and Post groups, abundances of the genus , phylum and phylum were markedly increased and that of the genus and phylum were decreased.
The results suggested a strong association of these bacteria with OSCC. Especially, phylum was significantly associated with early recurrence of OSCC. Thus, oral microbiome analysis may have a potential of novel OSCC detection and prognostic tool.
近年来，口腔微生物群与口腔鳞状细胞癌（OSCC）的发生和发展相关的可能性受到关注；然而，这种关联仍不明确。在此，我们使用下一代测序全面分析唾液样本的微生物群谱，随后确定口腔微生物群谱与OSCC之间的关联。
分析OSCC患者、口腔白斑（OLK）患者及OSCC术后（Post）患者唾液样本中的微生物群谱。通过基于这些微生物群谱比较各组的细菌多样性和相对丰度，鉴定与OSCC相关的候选细菌，并评估它们作为OSCC检测工具的适用性。
唾液样本各组间属丰度（ 、 和 ）存在显著差异。在OSCC组中，与OLK组和Post组相比， 属、 门和 门的丰度显著增加，而 属和 门的丰度降低。
结果表明这些细菌与OSCC密切相关。特别是， 门与OSCC的早期复发显著相关。因此，口腔微生物群分析可能具有作为新型OSCC检测和预后工具的潜力。
