Ahmad Saad, Jayamanne Dasantha, Bergamin Sarah, Lawless Anna, Guminski Alexander, Lee Adrian, Yuile Alexander, Wheeler Helen, Eade Thomas, Back Michael, Molloy Mark, Oh Byeongsang
Faculty of Medicine and Health, University of Sydney, Sydney, NSW 2006, Australia.
Northern Sydney Cancer Centre, Royal North Shore Hospital, St Leonards, NSW 2065, Australia.
Cancers (Basel). 2025 Aug 15;17(16):2667. doi: 10.3390/cancers17162667.
: The oral microbiome has been implicated in the pathogenesis of head and neck squamous cell carcinoma (HNSCC). This review examines the association between specific oral bacterial taxa and HNSCC. : A systematic review was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines to examine the relationship between the oral microbiome and HNSCC. A comprehensive literature search was conducted in databases including EMBASE, Cochrane Library, Web of Science, Medline, and PubMed. : Following the screening of 284 articles, 21 studies met the inclusion criteria, comprising 1023 HNSCC patients (male: n = 806, 79%; female: n = 217, 21%) and 837 healthy controls (male: n = 622, 74%; female: n = 215, 25.7%). Although findings on alpha diversity were inconsistent, a significant difference in beta diversity was consistently reported between HNSCC patients and healthy controls. HNSCC patients exhibited higher relative abundances of and at the phylum level; , , , , and at the genus level; and , , spp., and at the species level. In contrast, healthy controls showed higher abundances of and at the phylum level; , , , , and at the genus level; and , , and at the species level in most studies. : The findings indicate distinct alterations in oral microbiome diversity and composition among HNSCC patients, highlighting the role of microbial dysbiosis in cancer progression. Standardized protocols for oral sample collection and microbiota analysis are essential to facilitate more robust, comparable, and clinically meaningful research outcomes.
口腔微生物群与头颈部鳞状细胞癌（HNSCC）的发病机制有关。本综述探讨了特定口腔细菌类群与HNSCC之间的关联。：按照系统评价和Meta分析的首选报告项目（PRISMA）指南进行了一项系统评价，以研究口腔微生物群与HNSCC之间的关系。在包括EMBASE、Cochrane图书馆、科学网、Medline和PubMed在内的数据库中进行了全面的文献检索。：在筛选了284篇文章后，21项研究符合纳入标准，包括1023例HNSCC患者（男性：n = 806，79%；女性：n = 217，21%）和837名健康对照者（男性：n = 622，74%；女性：n = 215，25.7%）。尽管关于α多样性的研究结果不一致，但HNSCC患者和健康对照者之间β多样性的显著差异一直被报道。HNSCC患者在门水平上表现出较高相对丰度的 和 ；在属水平上表现出较高相对丰度的 、 、 、 和 ；在种水平上表现出较高相对丰度的 、 、 spp.和 。相比之下，在大多数研究中，健康对照者在门水平上表现出较高丰度的 和 ；在属水平上表现出较高丰度的 、 、 、 和 ；在种水平上表现出较高丰度的 、 、 和 。：研究结果表明HNSCC患者口腔微生物群的多样性和组成存在明显改变，突出了微生物失调在癌症进展中的作用。标准化的口腔样本采集和微生物群分析方案对于促进更可靠、可比且具有临床意义的研究结果至关重要。
