Hosseini Seyed Mostafa, Farmany Abbas, Arabestani Mohammad Reza
Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Dental Research Center, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran.
Biol Trace Elem Res. 2020 Apr;194(2):463-471. doi: 10.1007/s12011-019-01798-0. Epub 2019 Jul 11.
Disease relapse is one of the most important challenges in treating brucellosis. The aim of this study was to investigate the effect of doxycycline-loaded solid nanoparticles (DOX-SLN) on acute and chronic brucellosis, serum levels of trace elements, and biochemical and hematological parameters in rats infected with Brucella melitensis. The treating effect of DOX-SLN was evaluated by investigating serum levels of trace elements such as zinc (Zn), copper (Cu), iron (Fe), magnesium (Mg), calcium (Ca), phosphorus (P), sodium (Na), and potassium (K); biochemical parameters such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total bilirubin (TBil); and hematological parameters in rats suffering from acute and chronic brucellosis. The mean size, zeta potential, PDI, drug loading, and encapsulation efficiency of the synthesized nanoparticles were 299 ± 34 nm, - 28.7 ± 3.2 mV, 0.29 ± 0.027, 11.2 ± 1.3%, and 94.9 ± 3.2%, respectively. The serum level of trace elements (Zn, Cu, Fe), biochemical parameters (AST, ALT, ALP, TBil), and hematological parameters (white blood cell (WBC) and hemoglobin (HB)) were significantly different between healthy and infected rats. DOX-SLN had an appropriate treating effect on chronic brucellosis through modifying the serum level of mentioned factors. Assessing the serum level of trace elements, biochemical and hematological parameters can be useful in detecting brucellosis with other diagnostic tests and determining its acute or chronic phase. DOX-SLN had a better efficacy in treating chronic brucellosis than ordinary free drugs used routinely in this regard.
疾病复发是布氏杆菌病治疗中最重要的挑战之一。本研究的目的是调查载多西环素固体纳米粒（DOX-SLN）对感染羊种布鲁氏菌的大鼠急性和慢性布氏杆菌病、血清微量元素水平以及生化和血液学参数的影响。通过调查血清中锌（Zn）、铜（Cu）、铁（Fe）、镁（Mg）、钙（Ca）、磷（P）、钠（Na）和钾（K）等微量元素水平；丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、碱性磷酸酶（ALP）和总胆红素（TBil）等生化参数；以及患有急性和慢性布氏杆菌病大鼠的血液学参数，来评估DOX-SLN的治疗效果。合成纳米粒的平均粒径、ζ电位、多分散指数、载药量和包封率分别为299±34nm、-28.7±3.2mV、0.29±0.027、11.2±1.3%和94.9±3.2%。健康大鼠和感染大鼠之间血清微量元素（Zn、Cu、Fe）水平、生化参数（AST、ALT、ALP、TBil）和血液学参数（白细胞（WBC）和血红蛋白（HB））存在显著差异。DOX-SLN通过改变上述因子的血清水平对慢性布氏杆菌病有适当的治疗效果。评估血清微量元素水平、生化和血液学参数有助于通过其他诊断测试检测布氏杆菌病并确定其急性期或慢性期。在治疗慢性布氏杆菌病方面，DOX-SLN比常规使用的普通游离药物疗效更好。
