Infectious Disease Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Department of Microbiology, Faculty of Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Sci Rep. 2023 Aug 28;13(1):14012. doi: 10.1038/s41598-023-41150-0.
Can brucellosis-related biochemical and immunological parameters be used as diagnostic and treatment indicators? The goal of this project was to look at biochemical parameters, trace elements, and inflammatory factors in the acute and chronic stages of brucellosis after treatment with streptomycin and hydroxychloroquine-loaded solid lipid nanoparticles (STR-HCQ-SLN). The double emulsion method was used for the synthesis of nanoparticles. Serum levels of trace elements, vitamin D, CRP, and biochemical parameters were measured in rats involved in brucellosis. The therapeutic effect of STR-HCQ-SLN was compared with that of free drugs. In both healthy and infected rats, serum concentrations of copper, zinc, iron, magnesium, potassium, and biochemical parameters of the liver were significantly different. By altering the serum levels of the aforementioned factors, treatment with STR-HCQ-SLN had a positive therapeutic effect on chronic brucellosis. Vitamin D levels declined (46.4%) and CRP levels rose (from 7.5 mg to less than 1 mg) throughout the acute and chronic stages of brucellosis. This study showed that by comparing the biochemical parameters and the levels of trace elements in the serum of healthy and diseased mice in the acute and chronic stages of brucellosis, it is possible to get help from other routine methods for diagnosis.
布鲁氏菌病相关的生化和免疫学参数能否作为诊断和治疗指标？本项目旨在观察链霉素和载羟氯喹固体脂质纳米粒（STR-HCQ-SLN）治疗后布鲁氏菌病急、慢性期的生化参数、微量元素和炎症因子。采用复乳法合成纳米粒。检测布鲁氏菌病大鼠血清微量元素、维生素 D、CRP 和生化参数。比较 STR-HCQ-SLN 与游离药物的治疗效果。在健康和感染大鼠中，铜、锌、铁、镁、钾的血清浓度和肝脏的生化参数均有显著差异。通过改变上述因素的血清水平，STR-HCQ-SLN 对慢性布鲁氏菌病有积极的治疗作用。维生素 D 水平下降（46.4%），CRP 水平升高（从 7.5mg 降至 1mg 以下），贯穿布鲁氏菌病急、慢性期。本研究表明，通过比较布鲁氏菌病急、慢性期健康和患病小鼠血清中的生化参数和微量元素水平，可以为其他常规诊断方法提供帮助。
