一种由 GO 复合纤维和上转换 MSNs 组成的柔性智能膜，用于 microRNA 检测。

A flexible smart membrane consisting of GO composite fibres and upconversion MSNs for microRNA detection.

机构信息

State Key Laboratory of Silicon Materials, School of Materials Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, People's Republic of China.

出版信息

Chem Commun (Camb). 2019 Jul 30;55(62):9104-9107. doi: 10.1039/c9cc02907a.

DOI:10.1039/c9cc02907a

PMID:31298232

Abstract

We have developed a photoluminescent membrane for microRNA detection, consisting of chemically modified mesoporous silica nanoparticles (CaF2:Yb/Ho@MSNs) attached, via single stranded DNA probes, to flexible polyurethane fibres coated with graphene oxide (GO). By detecting the release of the luminescent nanoparticles resulting from complementary co-hybridization between target miRNA sequences and the DNA probe, accurate measurements of the miRNA concentration at high sensitivity levels can be obtained. The constructs therefore offer a route to rapid detection and the potential for early cancer diagnosis.

摘要

我们开发了一种用于 microRNA 检测的光致发光膜，它由通过单链 DNA 探针附着的化学修饰的介孔硅纳米粒子（CaF2:Yb/Ho@MSNs）组成，该探针附着在涂有氧化石墨烯（GO）的柔性聚氨酯纤维上。通过检测目标 miRNA 序列与 DNA 探针之间互补共杂交导致的发光纳米粒子的释放，可以在高灵敏度水平上获得 miRNA 浓度的准确测量。因此，这些构建物提供了快速检测的途径，并有可能进行早期癌症诊断。