Université de Lille, Faculté de Médecine, CHU de Lille, Laboratoire de Virologie/EA3610, F-59037, Lille, France.
Université de Monastir, Faculté de Pharmacie, Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives LR99ES27, Monastir, Tunisia.
Sci Rep. 2019 Jul 12;9(1):10080. doi: 10.1038/s41598-019-46227-3.
Coxsackieviruses B (CV-B) belong to the EV-B species. CV-B and particularly CV-B4 are thought to be involved in the development of chronic diseases like type 1 diabetes (T1D). The mechanisms of the enteroviral pathogenesis of T1D are not well known, yet. The in vitro studies are rich with information but in vivo infection models are needed to investigate the impact of viruses onto organs. Our objective was to study the impact of CV-B4E2 combined with a single sub-diabetogenic dose of streptozotocin (STZ) on the pancreas of mice. The infection with CV-B4E2 of CD1 outbred mice treated with a sub-diabetogenic dose of STZ induced hyperglycemia and hypoinsulinemia. Along with the chemokine IP-10, viral RNA and infectious particles were detected in the pancreas. The pancreas of these animals was also marked with insulitis and other histological alterations. The model combining STZ and CV-B4E2 opens the door to new perspectives to better understand the interactions between virus and host, and the role of environmental factors capable, like STZ, to predispose the host to the diabetogenic effects of enteroviruses.
柯萨奇病毒 B（CV-B）属于 EV-B 种。CV-B，特别是 CV-B4，被认为与 1 型糖尿病（T1D）等慢性疾病的发展有关。然而，肠病毒 T1D 发病机制的机制尚不清楚。体外研究信息丰富，但需要体内感染模型来研究病毒对器官的影响。我们的目的是研究 CV-B4E2 与单次亚致糖尿病剂量链脲佐菌素（STZ）联合对小鼠胰腺的影响。用亚致糖尿病剂量 STZ 处理的 CD1 远交系小鼠感染 CV-B4E2 可诱导高血糖和胰岛素血症。趋化因子 IP-10、病毒 RNA 和感染性颗粒在胰腺中被检测到。这些动物的胰腺也有胰岛炎和其他组织学改变的特征。STZ 和 CV-B4E2 联合的模型为更好地理解病毒和宿主之间的相互作用以及环境因素（如 STZ）在使宿主易患肠病毒致糖尿病作用方面的作用开辟了新的视角。
