Department of Otolaryngology, University of Colorado School of Medicine, Aurora, CO, USA.
Department of Cell and Developmental Biology, University of Colorado School of Medicine, Aurora, CO, USA.
Chem Senses. 2019 Sep 7;44(7):511-521. doi: 10.1093/chemse/bjz046.
Mucins are a key component of the surface mucus overlying airway epithelium. Given the different functions of the olfactory and respiratory epithelia, we hypothesized that mucins would be differentially expressed between these 2 areas. Secondarily, we evaluated for potential changes in mucin expression with radiation exposure, given the clinical observations of nasal dryness, altered mucus rheology, and smell loss in radiated patients. Immunofluorescence staining was performed to evaluate expression of mucins 1, 2, 5AC, and 5B in nasal respiratory and olfactory epithelia of control mice and 1 week after exposure to 8 Gy of radiation. Mucins 1, 5AC, and 5B exhibited differential expression patterns between olfactory and respiratory epithelium (RE) while mucin 2 showed no difference. In the olfactory epithelium (OE), mucin 1 was located in a lattice-like pattern around gaps corresponding to dendritic knobs of olfactory sensory neurons, whereas in RE it was intermittently expressed by surface goblet cells. Mucin 5AC was expressed by subepithelial glands in both epithelial types but to a higher degree in the OE. Mucin 5B was expressed by submucosal glands in OE and by surface epithelial cells in RE. At 1-week after exposure to single-dose 8 Gy of radiation, no qualitative effects were seen on mucin expression. Our findings demonstrate that murine OE and RE express mucins differently, and characteristic patterns of mucins 1, 5AC, and 5B can be used to define the underlying epithelium. Radiation (8 Gy) does not appear to affect mucin expression at 1 week.
N/A (Basic Science Research).IACUC-approved study [Protocol 200065].
粘蛋白是覆盖气道上皮的表面粘液的主要成分。鉴于嗅觉和呼吸上皮的不同功能，我们假设这些粘蛋白在这两个区域会有不同的表达。其次，鉴于放射治疗患者的鼻腔干燥、粘液流变学改变和嗅觉丧失的临床观察，我们评估了粘蛋白表达的潜在变化。免疫荧光染色用于评估对照小鼠和暴露于 8Gy 辐射后 1 周的鼻呼吸和嗅觉上皮中粘蛋白 1、2、5AC 和 5B 的表达。粘蛋白 1、5AC 和 5B 在嗅觉上皮 (OE) 和呼吸上皮 (RE) 之间表现出不同的表达模式，而粘蛋白 2 没有差异。在嗅觉上皮 (OE) 中，粘蛋白 1 呈晶格状模式，围绕对应于嗅觉感觉神经元树突棘的间隙，而在 RE 中，它由表面杯状细胞间歇性表达。粘蛋白 5AC 在两种上皮类型的上皮下腺中表达，但在 OE 中表达程度更高。粘蛋白 5B 在 OE 的粘膜下腺和 RE 的表面上皮细胞中表达。在单次 8Gy 照射后 1 周，粘蛋白表达未观察到定性影响。我们的研究结果表明，鼠 OE 和 RE 表达的粘蛋白不同，粘蛋白 1、5AC 和 5B 的特征模式可用于定义潜在的上皮组织。辐射（8Gy）在 1 周内似乎不会影响粘蛋白表达。
N/A（基础科学研究）。已批准的 IACUC 研究[协议 200065]。
