Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.
Research Group for Faculty of Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen, 40002, Thailand.
Appl Biochem Biotechnol. 2020 Jan;190(1):57-72. doi: 10.1007/s12010-019-03094-y. Epub 2019 Jul 13.
To address the high demand for Pueraria candollei var. mirifica (PM) used as the active ingredient in health products and its difficulty to cultivate in the field, the growth and production of deoxymiroestrol (DME) and isoflavonoid (ISF) phytoestrogens in PM cell suspensions were studied. In a 125-mL shake flask, the cell suspension produced DME [78.7 ± 8.79-116 ± 18.2 μg/g dry weight (DW)] and ISF (140 ± 6.83-548 ± 18.5 μg/g DW), which are the predominant ISF glycosides. While ISF aglycones accumulated in the PM cell suspension cultured in the airlift bioreactor. The DME content was increased to 976 ± 79.6 μg/g DW when the PM cell suspension was cultured in the 5-L scale bioreactor. The production of DME and ISF was enhanced by elicitors including methyl jasmonate (MJ), yeast extract (YE), and chitosan (CHI). MJ produced the highest induction of DME accumulation, while ISF accumulation was the highest with YE treatment. Analysis of catalase activity implied that the elicitors enhanced ROS production, which resulted in the enhancement of DME and ISF production and accumulation in PM cell suspension cultures. PM cell suspension culture is a promising source of beneficial PM phytoestrogens that exhibit bioactivity that may useful for the treatment of menopausal symptoms.
为满足作为保健品活性成分的 Pueraria candollei var. mirifica（PM）的高需求，以及其在田间难以种植的问题，研究了 PM 细胞悬浮液中脱氧米古酚（DME）和异黄酮（ISF）植物雌激素的生长和生产。在 125-mL 摇瓶中，细胞悬浮液产生 DME [78.7±8.79-116±18.2μg/g 干重（DW）]和 ISF（140±6.83-548±18.5μg/g DW），这是主要的 ISF 糖苷。而异黄酮苷元在气升式生物反应器中培养的 PM 细胞悬浮液中积累。当 PM 细胞悬浮液在 5-L 规模的生物反应器中培养时，DME 的含量增加到 976±79.6μg/g DW。茉莉酸甲酯（MJ）、酵母提取物（YE）和壳聚糖（CHI）等诱导剂可提高 DME 和 ISF 的产量。MJ 对 DME 积累的诱导作用最高，而 YE 处理时 ISF 积累最高。过氧化氢酶活性分析表明，诱导剂增强了 ROS 的产生，从而促进了 PM 细胞悬浮培养中 DME 和 ISF 的生产和积累。PM 细胞悬浮培养是一种有前途的有益 PM 植物雌激素的来源，其生物活性可能对治疗更年期症状有用。
