Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal.
CNR, Istituto di Ricerca Sulle Acque, Via F. De Blasio 5, 70132 Bari, Italy.
Sci Total Environ. 2019 Nov 10;690:739-747. doi: 10.1016/j.scitotenv.2019.06.461. Epub 2019 Jun 28.
The occurrence of pharmaceuticals in the environment is a topic of concern. Carbamazepine (CBZ) is a widespread antiepileptic drug and due to its physical-chemical characteristics minimal removal is achieved in conventional water treatments, and thus has been suggested as a molecular marker of wastewater contamination in surface water and groundwater. The present study reports the biotransformation of CBZ by the bacterial strain Labrys portucalensis F11. When supplied as a sole carbon source, a 95.4% biotransformation of 42.69 μM CBZ was achieved in 30 days. In co-metabolism with acetate, complete biotransformation was attained at a faster rate. Following a target approach, the detection and identification of 14 intermediary metabolites was achieved through UPLC-QTOF/MS/MS. Biotransformation of CBZ by the bacterial strain is mostly based on oxidation, loss of -CHNO group and ketone formation reactions; a biotransformation pathway with two routes is proposed. The toxicity of untreated and treated CBZ solutions was assessed using Vibrio Fischeri and Lepidium sativum acute toxicity tests and Toxi-Chromo Test. The presence of CBZ and/or its degradations products in solution resulted in moderate toxic effect on Vibrio Fischeri, whereas the other organisms were not affected. To the best of our knowledge this is the first report that proposes the metabolic degradation pathway of CBZ by a single bacterial strain.
环境中药物的存在是一个令人关注的问题。卡马西平（CBZ）是一种广泛使用的抗癫痫药物，由于其物理化学特性，在常规水处理中几乎无法去除，因此被认为是地表水和地下水中废水污染的分子标志物。本研究报告了细菌菌株 Labrys portucalensis F11 对 CBZ 的生物转化。当作为唯一的碳源供应时，在 30 天内可实现 42.69 μM CBZ 的 95.4%生物转化。在共代谢中添加乙酸盐时，可更快地达到完全生物转化。通过靶向方法，通过 UPLC-QTOF/MS/MS 检测和鉴定了 14 种中间代谢产物。细菌菌株对 CBZ 的生物转化主要基于氧化、-CHNO 基团的损失和酮形成反应；提出了一条具有两条途径的生物转化途径。使用发光细菌和萝卜种子急性毒性试验和 Toxi-Chromo 试验评估未经处理和处理的 CBZ 溶液的毒性。在溶液中存在 CBZ 和/或其降解产物会对发光细菌产生中等毒性作用，而其他生物体不受影响。据我们所知，这是首次报道单一细菌菌株对 CBZ 的代谢降解途径。
